DNA Barcoding of Ricinus communis from Different Geographical Origin by Using Chloroplast matK and Internal Transcribed Spacers


Ricinus communis have attracted considerable attention because of its specific industrial and pharmacological activities. DNA barcodes can be used as reliable tools to facilitate the identification of medicinal plants for the safe use, quality control and forensic investigation. In this study, the differential identification of eight accessions of R. com-munis was investigated through DNA sequence analysis of two candidate DNA barcodes. The nucleotide sequence of internal transcribed spacers (ITS2) and chloroplast maturase gene (matK) have been determined to construct the phylogenetic tree. The phylogenetic relationships of accessions based on the nrITS2 region and partial matK region showed that all accessions in this study were related to three geographical origins. Based on sequence align-ment and phylogenetic analyses we concluded that the ITS2 sequences can distinguish R. communis accessions from different geographical distributions.

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M. Enan, M. Al-Deeb, N. Fawzy and K. Amiri, "DNA Barcoding of Ricinus communis from Different Geographical Origin by Using Chloroplast matK and Internal Transcribed Spacers," American Journal of Plant Sciences, Vol. 3 No. 9, 2012, pp. 1304-1310. doi: 10.4236/ajps.2012.39157.

Conflicts of Interest

The authors declare no conflicts of interest.


[1] B. Christopher, “The Royal Horticultural Society A-Z Encyclopedia of Garden Plants,” Dorling Kindersley, London, 1996, pp. 884-885.
[2] A. Scarpa and A. Guerci, “Various Uses of the Castor Oil Plant (Ricinus communis L.), A Review,” Journal of Ethnopharmacology, Vol. 5, No. 2, 1982, pp. 117-137. doi:10.1016/0378-8741(82)90038-1
[3] M. F. Jose and V. Leonardo, “Castor,” In: S. K. Gupta, Ed., Technological Innovations in Major World Oil Crops, Volume 1: Breeding, Springer, 2012, pp. 237-265.
[4] W. J. Kress, K. J. Wurdack, E. A. Zimmer, L. A. Weigt and D. H. Janzen, “Use of DNA Barcodes to Identify Flowering Plants,” Proceedings of the National Academy of Sciences USA, Vol. 102, 2005, pp. 8369-8374. doi:10.1073/pnas.0503123102
[5] W. J. Kress and D. L. Erickson, “A Two-Locus Global DNA Barcode for Land Plants: The Coding rbcL Gene Complements the Non-Coding trnH-psbA Spacer Region,” PloS One, Vol. 2, 2007, p. e508. doi:10.1371/journal.pone.0000508
[6] CBOL Plant Working Group, “A DNA Barcode for Land Plants,” Proceedings of the National Academy of Sciences USA, Vol. 106, 2009, pp. 12794-12797. doi:10.1073/pnas.0905845106
[7] S. Chen, H. Yao, J. Han, C. Liu, J. Song, L. Shi, Y. Zhu, X. Ma, T. Gao, X. Pang, K. Luo, Y. Li, X. Li, X. Jia, Y. Lin and C. Leon, “Validation of ITS2 Region as a Novel DNA Barcode for Identifying Medicinal Plant Species,” PLoS One, Vol. 5, 2010, p. e8613. doi:10.1371/journal.pone.0008613
[8] H. Yao, J. Song, C. Liu, K. Luo, J. Han, Y. Li, X. Pang, H. Xu, Y. Zhu, P. Xiao and S. Chen, “Use of ITS2 Region as the Universal DNA Barcode for Plants and Animals,” PLoS One, Vol. 5, No. 10, 2010, p. e13102. doi:10.1371/journal.pone.0013102
[9] E. M. Soininen, A. Valentini, E. Coissac, C. Miquel, L. Gielly, C. Brochmann, A. K. Brysting, J. H Sonstebo, R. A. Ims, N. G. Yoccoz and P. Taberlet, “Analyzing Diet of Small Herbivores: The Efficiency of DNA Barcoding Coupled with High-Throughput Pyrosequencing for Deciphering the Composition of Complex Plant Mixtures,” Frontier in Zoology, Vol. 6, 2009, p. 16. doi:10.1186/1742-9994-6-16
[10] M. Stech, E. Kolvoort, M. J. J. E Loonen, K. Verieling and J. D. Kruijer, “Bryophyte DNA Sequences from Faeces of an Arctic Herbivore, Barnacle Goose (Branta leucopsis),” Molecular Ecology Resources, Vol. 11, No. 2, 2011, pp. 404-408. doi:10.1111/j.1755-0998.2010.02938.x
[11] K. M. Evans, A. H. Wortley and D. G. Mann, “An Assessment of Potential Diatom ‘Barcode’ Genes (cox1, rbcL, 18S and ITS rDNA) and Their Effectiveness in Determining Relationships in Sellaphora (Bacillariophyta),” Protist, Vol. 158, No. 3, 2007, pp. 349-364. doi:10.1016/j.protis.2007.04.001
[12] P. D. Hebert, S. Ratnasingham and J. R. deWaard, “Barcoding Animal Life: Cytochrome c Oxidase Subunit 1 Divergences among Closely Related Species,” Proceedings of the Royal Society B: Biological Sciences, Vol. 270, 2003, pp. 95-99.
[13] M. Hajibabaei, G. A. C. Singer, P. D. N. Hebert and D. A. Hickey, “DNA Barcoding: How It Complements Taxonomy, Molecular Phylogenetics and Population Genetics,” Trends in Genetics, Vol. 23, No. 4, 2007, pp. 167-172. doi:10.1016/j.tig.2007.02.001
[14] R. S. Cowan, M. W. Chase, W. J. Kress and V. Savolainen, “300,000 Species to Identify: Problems, Progress, and Prospects in DNA Barcoding of Land Plants,” Taxon, Vol. 55, No. 3, 2006, pp. 611-616.

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