Investigation of an inflammatory viral disease HBV in cardiac patients through polymerase chain reaction


In the present study 100 cardiac patients were randomly selected from the cardiology ward, Allied Hospital Faisalabad, Pakistan. All the selected cases were analysed for different parameters like Hepatitis B surface Antigen (HbsAg), Bilirubin, Alkaline phosphatase, serum glutamic pyruvic transaminase, and serum glutamic-oxaloacetic transaminase. Out of total 16% patients were lying in the age of 21-30 year, 25% in the age of 31-40 year, 35% in the age of 41-50 year, 19% in the age of 51-60 years and 5% patients in the age of 61-70 years. No subject was found positive in age group 21-30 years patients. 35% patients have higher value of SGPT while, other 26% were with higher value of SGOT. Rest of the 32 and 24% have higher ALP and Bilirubin levels, respectively. Assay profile revealed that ALP level was increased with increasing age, body mass index, Creactive protein, diabetes, smoking, sex, serum uric acid, lead, cadmium, hypercholesterolemia, lesion of liver and cardiovascular disease. The serum of the eight HbsAg positive cases were tested for the presence of HBV through PCR and no sample was found positive. At the end of the study, PCR amplified samples were run on 1.5% agarose gel to confirm the case.

Share and Cite:

Saher, F. , Rehman, K. , Qureshi, J. , Irshad, M. and Iqbal, H. (2012) Investigation of an inflammatory viral disease HBV in cardiac patients through polymerase chain reaction. Advances in Bioscience and Biotechnology, 3, 417-422. doi: 10.4236/abb.2012.324059.

Conflicts of Interest

The authors declare no conflicts of interest.


[1] Liu, B., Li, J., Han, Y., Liu, Y., Kong, L., Cao, Y. and Huang, Z. (2010) Dynamic analysis of lymphocyte subsets of peripheral blood in patients with acute self-limited hepatitis B. Health 2, 736-741.
[2] WHO. (1997) Emerging and other communicable diseases (EMC) weekly Epimiological report (WER). 72, 5487-5494.
[3] Noah, D.N., Njouom, R., Bonny, A., Pirsou., Meli, J. and Sida, M.B. (2011) HBs antigene prevalence in blood donors and the risk of transfusion of hepatitis B at the central hospital of yaounde, Cameroon. Open J. Gastroenterol. 1, 23-27.
[4] Badar, N., Farooq, U., Ali, S., Nisar, N., Abubakar, M. and Qureshi, J.A. (2012) A Molecular Approach for Genotyping of Hepatitis B Virus Using Restriction Pattern Analysis of S Amplicon in Pakistan. Open J. Med. Microbiol. 2, 16-23.
[5] Lavenchy, D. (2004) Hapetitis B virus epidemiology, disease burden, treatment, and current and emerging prevention and control measures. J. Viral Hepatitis. 11, 97-107.
[6] Schirmbeck, R., Zheng, X., Roggendorf, M., Geisserler, M., Chiisari, F.V., Reimann, J. and Lu, M. (2001) Targeting immune responses to selected T cells or antibody defined determinants of hepatitis B virus surface antigen by plasmid DNA vaccines encoding chimeric antigen. J. Immunol. 166, 1405-1413.
[7] Carman, M.H., Chen, C.J. and Lai, M.S. (1997) Universal Hepatitis B Vaccination in Taiwan and the incidence of hepatocellular carcinoma in Childhood Hepatona study Group’s. Eng. J. Med. 336, 1855-1859.
[8] Kao, J.H., Chen, P.J., Lai, M.Y. and Chen, D.S. (2000) Hepatitis B genotypes correlate with clinical outcomes in patients with chronic hepatitis B. Gasteroenterol. 118, 554-559.
[9] Sambrook, J., Fritsch, E.F. and Maniatis, T. (1999) Molecular cloning, A Laboratory Mannual, 2nd Edition, Gold Spring Harbor Laboratory Press, Cold spring Harbour New York.
[10] Khan, A.A., Parveen, N., Mahaboob, V.S., Rajendraprasad, A., Ravindraprakash, H.R., Venkateswarlu, J., Rao, P., Pande, G., Narusu, M.L., Khaja, M.N., Pramila, R., Habeeb, A. and Habibullah, C.M. (2008) Management of hyperbilirubinemia in biliary atresia by hepatic progenitor cell transplantation through hepatic artery: a case report. Transplant Proc. 40, 1153-1155.
[11] Tsang, P.S., Trinh, H., Garcia, R.T., Phan, J.T., Ha, N.B., Nguyen, H., Nguyen, K., Keeffe, E.B. and Nguyen, M.H. (2008) Significant prevalence of histologic disease in patients with chronic hepatitis B and mildly elevated serum alanine aminotransferase levels. Clin. Gastroenterol. He-patol. 6, 569-574.
[12] Chopra, C.G.S., Gupta, Lt. Col.P.K., Anand, Col.A.C., Varma, Col.P.P., Nai, Col.V. and Rai, Lt.Gen.R. (2005) Real Time-PCR HBV-DNA Analysis: Significance and First Experience in Armed Forces. MJAFI. 61, 234-237.
[13] Leung, N. (2002) Transmission of hepatitis B: case selection and duration of therapy. J. Gastroenterol. Hepatol. 17, 409-414.
[14] Fleming. (2000) The origin and evolution of hepatitis virus in humans. J. Gen. Virol. 82, 693-712.
[15] Rahman, M., Akhtar, G.N. and Lodi, Y. (2002) Transfusion transmitted HIV and HBV infection in Punjab, Pakistan. Pak. J. Med. Sci. 18, 18-25.

Copyright © 2023 by authors and Scientific Research Publishing Inc.

Creative Commons License

This work and the related PDF file are licensed under a Creative Commons Attribution 4.0 International License.