Malate dehydrogenase in plants: Its genetics, structure, localization and use as a marker

DOI: 10.4236/abb.2012.34053   PDF   HTML   XML   4,888 Downloads   7,980 Views   Citations


This article is an overview of literature data on the structure, properties, functions and genetic control of the enzyme malate dehydrogenase (MDH) in plants. In most of the plant entities studied, this enzyme is highly polymorphic, which means that malate dehydrogenase has multiple molecular forms. It has been found that MDH polymorphism in each species is genetically determined by several loci with multiple alleles. A readily identifiable phenotypic manifestation and a high activity of malate dehydrogenase in diverse organs and tissues make this enzyme a reliable and convenient genetic marker, which can effectively be used in special, ecological and population genetics.

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Yudina, R. (2012) Malate dehydrogenase in plants: Its genetics, structure, localization and use as a marker. Advances in Bioscience and Biotechnology, 3, 370-377. doi: 10.4236/abb.2012.34053.

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The authors declare no conflicts of interest.


[1] Hunter, R.L. and Markert, C.L. (1957) Histochemical demonstration of enzymes separated by zone electrophoresis in starch gel. Science, 125, No. 3261, 1294-1295.
[2] Markert, C.L. and Moller F. (1959) Multiple forms of enzymes: tissue, ontogenetic and species specific patterns. Proc. Natl. Acad. Sci., 45, 753-763.
[3] Dixson, M. and Webb, E.C. (1982) Enzymes. Mir,Moscow. (in Russ.).
[4] Shannon, L.M. (1968) Plant isoenzymes. Ann. Plant. Physiol. 19, 187-210.
[5] Scandalios. J.G. (1969) Genetic control of multiple forms of enzymes in plants; a review. Biochem. Genet., 3, No.1, 37-79.
[6] Longo, C.P. and Scandalios J.G. (1969) Nuclear gene control of mitochondrial malic dehydrogenase in maize. Proc. Natl. Acad. Sci., 62, No. 1, 104-11.
[7] Yang, N.S. and Scandalios J.G. (1974) Purification and biochemical properties of genetically defined malate dehydrogenase in maize. Arch. Biochem. Biophys., 161, 335-353.
[8] Yang, N.S. (1975) Biochemical properties and development expression of genetically determined malate dehydrogenase isozymes in maize. Isozymes. III Developmental Biology. N.Y. -San-Francisco-London: Acad. Press, 191-211.
[9] Lehninger, A.L. (1974) Biochemistry. Mir, Moscow. (in Russ.).
[10] Ting , I.P., Fuhr I., Curry R. and Zschoche W.G. (1975) Malate dehydrogenase isozymes in plant: preparation, properties and biological significance. Isozymes by ed. Markert, C.L.N.Y.-San-Francisco-London: Acad. Press. 369-384.
[11] Scandalios J.G. (1974) Isoenzymes in development and differentiation. Ann. Rev. Plant Physiol. 25. 225-258.
[12] Breidenbach, R.W. and Beevers H. (1967) Association of the glyoxylate cycle enzymes in a novel subcellular particle from castor bean endosperm. Biochem. Bio-phys. Res. Commun. 27, 462-469.
[13] Walk, R.A. and Hock B. (1977) Glyoxisomal and mitochondrial malate dehydrogenase of watermelon (Citrullus vulgaris) cotyledons. II Kinetic properties of the purified isoenzymes. Planta, 136, 221-228.
[14] Yamazaki, R.K. and Tolbert N.E. (1969) Malate dehydrogenase in leaf peroxisomes. Biochem. Biophys. Acta, 178, No.1, 11-20.
[15] Sadunishvili, T.A. and Nutsubidse, N.N. (1980) Intra cellular location of malate and glutamate dehydrogenases in lemon citruslimon leaves. Soobsheniya Akademii Nauk Gruzinskoy SSR, 100, No. 3, 693-696.
[16] Tarasova, R.S. (1988) Genetics and phenogenetics of malate dehydrogenase in sugar beet. Ph.D. Thesis, Institute of Cytology and Genetics, Novosibirsk (in Russ).
[17] Yudina, R.S. and Levites, E.V. (2007) Malate Dehydrogenase Isozymes as Markers of Organelles Physioligical State Sugar Beet (Beta vulgaris L.). Sugar Tech, 9, No. 1, 67-71.
[18] Yudina, R.S. and Levites, E.V. (2008) Subcellular Localization of Isozymes of NAD-dependent Malate Dehydrogenase in Sugar Beet (Beta vulgaris L.). Russ. J. Genet., 44, No.12, 1638-1643 (in Russ.).
[19] Gottlieb, L.D. (1982) Conservation and duplication of isozymes in plant. Science, 216, No. 4544, 373-380.
[20] Endo, T. and Morishma H. (1983) Rise. Isozymes in plant genetics and breeding. Amsterdam: Elsevier. Part B, 129-146.
[21] Orton, T.J. (1983) Celery and Celeriac (Apium graveolens L.) Isozymes in plant genetics and breeding. Amsterdam: Elsevier. Part B, 351-356.
[22] Torres, A.M. (1983) Fruit trees. Isozymes in plant genetics and breeding. Amsterdam: Elsevier. Part B., 401-421.
[23] Arus, P. and Orton, T.J. (1984) Inheritance patterns and linkage relationships of eight genes of celery (Apium graveolens). J. Heredity, 75, 11-14
[24] Yang, N.S. and Scandalios J.G. (1975a) Cytoplasmic synthesis of soluble and mitochondrial malate dehy-drogenase isozymes in maize. Arch. Biochem. Biophys, 171, 575-585.
[25] Yang, N.S. and Scandalios J.G. (1975b) De novo synthesis and developmental control of gene-controlled malate dehydrogenase isozymes in maize scutella. Biochem. Biophys. Acta, 384, 293-306.
[26] Yang, N.S., Sorenson, J.G. and Scandalios, J.G. (1977) Genetic control of mitochondrial malate dehydrogenase: evidence for duplicated chromosome segments. Proc. Natl.Acad. USA, 74, 310-314.
[27] McMillin, D.E. and Scandalios, J.G. (1980) Duplicated cytosolic malate dehydrogenase genes in Zea mays. Proc. Natl. Acad.Sci. USA. 77, 4866-4870.
[28] McMillin, D.E. and Scandalios, J.G. (1981) Genetic analysis of two groups of duplicated genes coding for mitochondrial malate dehydrogenase in Zea mays: Possible origin of mMdh genes by chromosomal segment duplication. Mol. Gen. Genet. 211-221.
[29] McMillin, D.E. and Scandalios, J.G. (1982) Genetic, immunological and gene dosage studies of mitochondrial and cytosolic MDH variante in maize. J. Heredity, 73, No. 3, 177-182.
[30] Goodman, M.M. and Stuber, C.W. (1983) Maize. Isozymes in plant genetics and breeding. Amsterdam: Elsevier. Part B. 1-33.
[31] Newton, K.J.(1983) Genetics of mitochondrial isozymes. Isozymes in plant and breeding. Amsterdam: Elsevier. Part A. 157-170.
[32] Goodman, M.M., Stuber, C.W., Lee, C.N. and Johnson, F.M. (1980) Genetics control of malate dehydrogenase isozymes in maize. Genetics, 94, 153-168.
[33] Taureilles-Saurel, C., Romieu, G., Robin, J-P. and Flanzy, C. (1975) Grape (Vitis vinifera L.) Malate Dehydrogenase. I. Intercellular Compartmentation of the Isoforms. Am.J.Enol.Vitic, 46, No. 1, 22-28.
[34] Walk, R.A., Michaeli, S. and Hock, B. (1977) Glyoxisomal and mitochond rial malate dehyddrogenase of watermelon (Citrullus vulgaris) cotyledons. I.Molecular properties of the purified isoenzymes. Planta, 136, 211-220.
[35] Gietl, C. and Hock, B. (1982) Organelle-bound malate dehydrogenase isoenzymes are synthe sized as higher molecular weight precursors. Plant Physiol. 70, No. 2, 483-487.
[36] Yudina, R.S., Zheleznova, N.B., Zakharova, O.V., Zheleznov, A.V., Shumny, V.K., (2005) Isozyme analysis in genetic collection of amaranths (Amaranthus L.). Russ. J. Genet., 41, No. 12, 1682-1687. (in Russ.).
[37] Torres, A.M. (1984) Isozymes from avocado cotyledons . J. Heredity, 75, 300-302.
[38] Powling, A., Islam, A.K.M.R., Sheferd, K.W. (1981) Isozymes in wheat-barley hybrid derivate lines. Biochem, Genet., 19, 237-254.
[39] Brown, A.H.D. and Munday, J. (1982) Population genetics structure and optimal sampling of land races of barley. Genetica, 40, 315-324.
[40] Linde-Laursen, I., Nelson, G. and Johansen, H.B. (1987) Distribution of isoenzyme markers at loci in a pedigree of European spring barley. Hereditas, 106, 241-251.
[41] Habig, W. and Racusen, D. (1968) A malate dehydrogenase of high molecular weight from bean leaves. Can. J. Bot., 719-720.
[42] Zoro, I.Bi., Maquet, A., Wathelet, B. and Baudoin, J.-P. (1999) Genetic control of isozymes in the primary gene pool Phaseolus lunatus L .Biotechnol. Agron. Soc. Environ, 3, No, 2, 10-27
[43] Harry, D.E. (1983) Identification of a Locus Modifjing the Electrophoretic Mobility of Malate Dehydrogenase Isozymes in Incense-Cedar (Calocedrus decurrens), and Its Implications for Population Studies. Biochemical Genetics, 21, Nos. 5/6, 417-434.
[44] Machado, M.F.P.S., Prioli, A.J. and Mangolin, C.A. (1993) Malate Dehydrogenase (MDH; EC1.1.1.37) Isozymes in Tissue and callus Cultures of Cereus peruvianus (Cactaceae) Biochemical Genetics, 31, Nos.3/4, 167-172.
[45] Torres, A.M., Soost, K.K., Mau-Lastovisks, T. (1978) Leaf isozymes as genetic markers in citrus. Amer. J. Bot., 68, No. 8, 869-881.
[46] Torres, A.M., Soost, K.K. and Diedenhoffen, U. (1982) Genetic and distinguishing nucellularfrom zygotic seedlings. J. Heredity, 73, No. 5, 335-339
[47] Nagi, A.N., Siddiqui, M.O., Kocsis, Z.G. and Vida,G. (1980) In vitro dissociation-recombination of malate dehydroganase subunits in Corydalis solida. Theor. Appl. Genet., 58, No. 2, 75-78.
[48] Ennos, R.A. (1986) Allozyme variation, linkage and duplication in the perrenial grass; Cynosurus cristatus J. Heredity, 77, 61-62
[49] Moran, G.F. and Bell, J.G. (1983) Eucalipthus. Isozymes in plant genetics and breeding. Amsterdam: Elsevier. Part B, 423-442.
[50] Prise, S. and Kahler, A.L. (1983) Oats. Isozymes in plant genetics and breeding. Amsterdam: Elsevier. Part B, 147-167.
[51] Arulsekar, S., Parfitt, D.E., Beres, W. and Hansche, P.E. (1986) Genetics of malate dehydrogenase isozymes in the peach. J. Heredity, 77, No. 1, 49-51.
[52] Marquard, R.D. and Skorpenske, R.G. (1989) Expression of heritable biochemical markers from various pecan tissues. Euphytica, 42, Nos. 1/2, 65-70.
[53] McLeod, M.J., Guttman, S.I. and Esbaugh, W.N. (1983) Peppers. Isozymes in plant genetics and breeding. Amsterdam: Elsevier. Part B, 189-201.
[54] Wijsman, N.J.W. (1983) Petunia. Isozymes in plant and breeding. Amsterdam; Elsevier. Part B. 229-252.
[55] Srtauss, S.N. and Conkle, M.T. (1986) Segregation, linkage and diversity of allozymes in knobcone (Pinus attenuata). Theor. Appl. Gen., 72, 483-493.
[56] Cheliak, W.M., Morgan, K., Dancik, B.P., Strobeck, C. and Yeeh, F.C.N. (1984) Segregationof allozymes in megagametophytes of viable seed from a natural population of jack pine, Pinus banksiana Lamb. Theor. Appl. Genet. 69, No. 2, 145-151.
[57] Guries, R.P. and Ledic, F.T. (1978) Inheritance of some polymorphic isozymes in pitch pine (Pinus rigida Mill.) Heredity, 40, 27-32.
[58] O’Malley,D.M., Guries, R.P. and Nordheim, E.V. (1986) Linkage analysis for 18 enzyme loci in Pinus rigida Mill. Theor. Appl. Genet. 72, 530-535.
[59] Sternberg,, L., Ting. I.P. and Hanscom, Z. (1979) Polymorphism of microbody malate dehydrogenase in Opuntia basilaris. Plant Physiol., 59, 329-330.
[60] Kiang, Y.T. and Gorman,M.B. (1983) Soybean. Isozymes in plant genetics and breeding. Amsterdam:Elsevier. Part B, 295-298.
[61] Vodenicharova, M.I., (1980) Genetics of isoenzymes in wheat species Uspekhi sovremennoi genetiki, 9, 171-182. (in Russ.).
[62] Benito, C. and Salinas, J. (1983) The chromosomal location of malate dehyd rogenase isozymes in hexaploid wheat (Triticum aestivum L.). Theor. Appl. Genet., 64, No. 3, 255-258.
[63] O’Sullivan, S.A. and Wedding, R.T. (1972) Malate dehydrogenase isoenzymes from cotton leaves . Molecular weight. Plant Physiol., 49, 117-123 .
[64] Newton, K.J. and Schwartz, D. (1980) Genetic basis of the major malate dehydrogenase isozymes in maize . Genetics, 95, 425-442.
[65] Cammaerts, D and Jacobs, M. (1975) Study of the intracellular location and the genetic control of malate dehydrogenase isozymes in Arabidopsis thaliana. Plant Sci. Lett.,4, No. 4, 249-256.
[66] Honold, G.R., Masko, V. and Stahman, M.A. (1967) NAD and NADP – dependent malate dehydrogenase in wheat. Naturwissenschaften, 54, 169.
[67] Scandalios, J.G. and Jang, N.S. (1973) Abstract. Genetics, 74, No. 2, 242.
[68] Tarasova, R.S. (1987) Genetically determined forms of NAD-dependent MDH and their use in biochemical passportization if inbred sugar beet lines. Genetica, 23, No. 9, 1630-1636. (in Russ.)
[69] Korochkin, L.I. (1977) Gene interaction in development. Nauka, Moscow (in Russ.)
[70] Khavkin, E.E. (1969) Formation of metabolic system in growing plant cells. Nauka, Novosibirsk (inRuss.)
[71] Sozinov, A.A. (1985) Protein polymorphism in genetic and selection. Nauka, Moscow (in Russ).
[72] Tarasova, R.S. Levites, E.V. and Maletsky, S.I. (1988) Isozyme as markers for identification of sugar beet inbred lines in the process of their development. Biochemical identification of varieties (Materials III International Symposium ISTA). Leningrad. USSR, 240-243.
[73] Yudina, R.S., Ibragimova, S.S. and Zheleznova, N.B. (2008) A study of the structure of amaranth (Amaranthus L.) populations using isozyme spectra. Vestnik VOGIS, 12, No. 3, 385-390 (in Russ.)
[74] Biruk, A. and Kazlovskya, Z. (2008)Prospects for using of isozymes markers in identification of apple cultivars. Sodiinkyste ir darzininkyste. Mokslo Darbai, 27(2), 359-364.

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