Comparative Phosphorus Removal Capabilities of Eurotium Herbarorium and Clostridium Species on Nigeria’S Agbaja Iron Ore


A study of phosphorus removal capabilities of Eurotium herbaroriumand Clostridium species from Nigeria’s Agbaja iron ore was carried out. Iron ore sample was crushed, sievedto obtain0.50 mm/0.25 mmparticle size distribution and cultured with mineral oil medium to facilitate microbial growth. Fungi and bacteria that concurrently grew weresubcultured in Sabouard dextrose agar and nutrient agar solutions that support fungal and bacterial growth, respectively,andcharacterized using standard procedures.Ore was exposed to these microbes to effect phosphorus removal in standard media and later analyzed at weekly interval using the standard volumetric ammonium phosphomolybdate method. The fermentation broth media were analyzed for iron, copper, cadmium, zinc, nickel, manganese and lead using the atomic absorption spectrophotometer. The microorganisms markedly removed phosphorus from the ore with 61.48% and 69.20%, respectively. For the funguspH remained in the acidic region and basic for the bacterium. Trace elements analyses of the initial and final orecontaining media recorded marked reduction in the concentration of these elements. A plausible explanation that is supported by literature is that the microorganisms accumulated them. This probably accounts for the drastic decrease in fungal biomass and bacterial density with the concomitant decrease in phosphorus removal observed towards the end.

Share and Cite:

O. Obot and C. Anyakwo, "Comparative Phosphorus Removal Capabilities of Eurotium Herbarorium and Clostridium Species on Nigeria’S Agbaja Iron Ore," Journal of Minerals and Materials Characterization and Engineering, Vol. 11 No. 8, 2012, pp. 848-852. doi: 10.4236/jmmce.2012.118077.

Conflicts of Interest

The authors declare no conflicts of interest.


[1] N. J. Amadi, A. A. Odunaike and G. P. Marthur, “Preliminary Bench Scale Beneficiation Studies with Three Lumps of Iron Ore Sample from Agbaja,” Technical Re-port, Central Metallurgical Research and Development Institute, Jos, 1982.
[2] G. G. O. O. Uwadiale, “Electrolytic Coagulation and Selective Flocculation of Agbaja Iron Ore,” Journal of Mining and Geology, Vol. 27, No. 1, 1991, pp. 77-85.
[3] G. G. O. O. Uwadiale and M. A. U. Nwoke, “Beneficiation of Agbaja Iron Ore by Reduction Roasting-Magnetic Separation: Semi Pilot Plant Scale-Up and Establishment of Residence Point of Phosphorus,” National Steel Council, Metallurgical Research and Tests Division, Jos, 1983.
[4] G. G. O. O. Uwadiale, “Beneficiation Studies of Agbaja Iron Ore,” Ph.D. Thesis, University of Strathclyde, Glasgow, 1984.
[5] G. G. O. O. Uwadiale and R. J. Whewell, “Effect of Temperature on Magnetizing Reduction of Agbaja Iron Ore,” Metallurgical and Materials Transaction B, Vol. 19, No. 5, 1988, pp. 731-735.
[6] V. Kudrin, “Steel Making,” MIR Publishers, Moscow, 1985, pp. 82-83.
[7] G. G. O. O. Uwadiale and O. Emeka, “Petrology of Ag-baja Ironstone, National Steel Council, Metallurgical Research and Tests Division,” Jos, 1983.
[8] P. Delvasto, et al., “Exploring the Possibilities of Biological Beneficiation of Iron-Ores: The Phosphorus Problem,” Proceedings of the 15th Steelmaking Conference, 5th Ironmaking Conference & 1st Environment and Recycling Symposium IAS (CD-ROM), Argentinean Steelmaking Institute (IAS), San Nicolás, 7-10 November 2005, pp. 71-82.
[9] N. Pradhan, et al., “Beneficiation of Iron Ore Slime Using Aspergillus niger and Bacillus circulans,” Bioresource Technology, Vol. 97, No. 15, 2006, pp. 1876-1879. doi:10.1016/j.biortech.2005.08.010
[10] C. N. Anyakwo and O. W. Obot, “Phosphorus Removal from Nigeria’s Agbaja Iron Ore by Aspergillus niger,” International Research Journal in Engineering, Science & Technology, Vol. 5, No. 1, 2008, pp. 54-58.
[11] H. L. Barnett and B. B. Hunter, “Illustrated Genera of Imperfect Fungi,” 4th Edition, Macmillan Publishing Coy, New York, Collier Macmillan Publishers, London, 1987.
[12] J. G. Holt, N. R. Krieg, P. H. A.Sneath, J. T. Stanley and S. T. Williams, “Bergy’s Manual of Determinative Bacteriology,” 9th Edition, Lippincott Williams and Wilkins, Maryland, 1994.
[13] S. K. Jain, “An Introduction to Metallurgical Analysis: Chemical and Instrumental,” Vikas Publishing House, New Delhi, 1982.
[14] P. K. Mohapatra, “Textbook of Environmental Microbiology,” I. K. International Publishing House Pvt Ltd., New Delhi, 2008.

Copyright © 2023 by authors and Scientific Research Publishing Inc.

Creative Commons License

This work and the related PDF file are licensed under a Creative Commons Attribution 4.0 International License.