In Vitro Evaluation of Gamma Irradiation on a Gel Formulation of Cratylia Mollis: Rheological Proporties and Microbiological Control

DOI: 10.4236/jcdsa.2012.22011   PDF   HTML     4,167 Downloads   7,081 Views   Citations


Lectin Cramoll-1,4, obtained from Cratylia mollis seeds (beans camaratu) was structurally characterized, biologically and pharmacologically, but its use as a biopharmaceutical is not well documented. The objective of this study is to propose a biopharmaceutical formulation lectin Cramoll-1,4, test their hemagglutinating properties in vitro as well as the use of gamma radiation as a continuous process of decontamination formulation. It was made of the extraction and purification Cramoll-1,4, was developed a gel formulation using Carbopol? as a vehicle, at concentrations of 50, 100, 200, 300 and 600 μg was irradiated with 60Co gamma rays in a dose of 7.549 kGy·h–1. The proposed formulation at a concentration of 300 μg produced an increase in the hemagglutinating units Cramoll-1,4 due to the synergistic effect caused by gamma radiation. Considering the diverse use of lectins, specific molecular and structural factors, as well as changes resulting from its formulation, concentration, irradiation and route of administration is of utmost importance to continue the studies in vitro, for subsequent application in vivo to characterize the physiological and molecular processes involved in the response and cellular effects.

Share and Cite:

M. Helena Madruga Lima-Ribeiro, R. Santos-Oliveira, M. Firmino de Santana, T. de Jesus Andreoli Pinto, I. Satiko Kikuchi, C. Gonçalves Mothé, L. Cassandra Breitenbach Barroso Coelho, M. Tereza dos Santos Correia and A. Maria dos Anjos Carneiro-Leão, "In Vitro Evaluation of Gamma Irradiation on a Gel Formulation of Cratylia Mollis: Rheological Proporties and Microbiological Control," Journal of Cosmetics, Dermatological Sciences and Applications, Vol. 2 No. 2, 2012, pp. 45-50. doi: 10.4236/jcdsa.2012.22011.

Conflicts of Interest

The authors declare no conflicts of interest.


[1] P. J. Houghton, et al., “In Vitro Tests and Ethnopharmacological Investigations: Wound Healing as an Example,” Journal of Ethnopharmacology, Vol. 100, No. 1-2, 2005, pp. 100-107. doi:10.1016/j.jep.2005.07.001
[2] N. Sharon and H. Lis, “A Century of Lectin Research (1888-1988),” Trends Biochemical Science, Vol. 12, 1987, pp. 483-487.
[3] M. T. S. Correia, L. C. B. B. Coelho, P. M. G. Paiva, “Lectins Carbohydrate Recognition Molecules: Are They Toxic?” In: Y. H. Siddique, Ed., Full name Recent Trends in Toxicology, Transworld Research Network, Kerala, 2008, pp. 47-59.
[4] J. Beuth, H. L. Ko, G. Pulverer, G. Uhlenbruck and H. Pichlmaier, “Importance of Lectins for the Prevention of Bacterial Infections and Cancer Metastases,” Glycoconjugate Journal, Vol. 12, No. 1, 1995, pp. 1-6. doi:10.1007/BF00731862
[5] M. Hong, A. Cassely, Y. Mechref and M. V. Novotny, “Sugar-Lectin Interactions Investigated through Affinity Capillary Electrophoresis,” Journal of Chromatography B: Biomedical Sciences and Applications, Vol. 752, No. 2, 2001, pp. 207-216. doi:10.1016/S0378-4347(00)00564-8
[6] P. M. G. Paiva and L. C. B. B. Coelho, “Purification and Partial Characterization of Two Lectin Isoforms from Cratylia mollis Mart (Camaratu Bean),” Applied Biochemistry and Biotechnology, Vol. 36, No. 2, 1992, pp. 113-119. doi:10.1007/BF02929691
[7] M. T. S. Correia and L. C. B. B. Coelho, “Purification of a Glucose/Manose Specific Lectin, Isoforma 1, from Seeds of Cratylia mollis Mart (Camaratu Bean),” Applied Biochemistry and Biotechnology, Vol. 55, No. 3, 1995, pp. 261-273. doi:10.1007/BF02786865
[8] G. A. De Souza, P. S. Oliveira, S. Trapani, A. C. Santos, J. C. Rosa, H. J. Laure, et al., “Amino Acid Sequence and Tertiary Structure of Cratylia mollis Seed Lectin,” Glycobiology, Vol. 13, No. 12, 2003, pp. 961-972. doi:10.1093/glycob/cwg115
[9] V. L. M Lima, M. T. S. Correia, Y. M. N. Cechinel, C. A. M. Sampaio, J. S. Owen, L. C. B. B. Coelho, “Immobilized Cratylia mollis Lectin as a Potential Matrix to Isolate Plasma Glycoproteins, including Lecithin-Cholesterol Acyltransferase,” Carbohydrate Polymers, 1997, Vol. 33, No. 6, pp. 27-32.
[10] E. I. Beltrao, M. T. Correia, J. Figueredo-Silva and L. C. Coelho, “Binding Evaluation of Isoform 1 from Cratylia mollis Lectin to Human Mammary Tissues,” Applied Biochemistry and Biotechnology, Vol. 74, No. 3, 1998, pp. 125-134. doi:10.1007/BF02825961
[11] P. M. Paiva, A. F. Souza, M. L. Oliva, J. F. Kennedy, M. S. Cavalcanti, L. C. Coelho, et al., “Isolation of a Trypsin Inhibitor from Echinodorus paniculatus Seeds by Affinity Chromatography on Immobilized Cratylia mollis Isolectins,” Bioresource Technology, Vol. 88, No. 1, 2003, pp. 75-79. doi:10.1016/S0960-8524(02)00272-9
[12] E. V. Maciel, V. S. Araújo-Filho, M. Nakazawa, Y. M. Gomes, L. C. Coelho and M. T. Correia, “Mitogenic Activity of Cratylia mollis Lectin on Human Lymphocytes,” Biologicals, Vol. 32, No. 1, 2004, pp. 57-60. doi:10.1016/j.biologicals.2003.12.001
[13] C. A. S. Andrade, M. T. S. Correia, L. C. B. B. Coelho, S. C. Nascimento and N. S. Santos-Magalhaes, “Antitumor Activity of Cratylia mollis Lectin Encapsulated into Liposomes,” Vol. 278, No. 2, 2004, pp. 435-445. doi:10.1016/j.ijpharm.2004.03.028
[14] M. D. L. Oliveira, “Estudo Bioeletroquímico de Nanosistemas Híbridos de Nanopartículas de Ouro E Lectinas Para O Desenvolvimento de Sensores,” Ph.D. Thesis, Federal University of Pernambuco, Recife, 2008.
[15] R. Angeli, N. V. N. da Paz, J. C. Maciel, F. F. B. Araújo, P. M. G. Paiva, G. M. T. Calazans, et al., “Ferromagnetic Levan Composite: An AffinityMatrix to Purify Lectin,” Journal of Biomedicine and Biotechnology, Vol. 2009, 2009, Ariticle ID: 179106. doi:10.1155/2009/179106
[16] T. Yasuoka, M. Sasaki, T. Fukunaga, T. Tsujikawa, Y. Fujiyama, R. Kushima, et al., “The Effects of Lectins on Indomethacin-Induced Small Intestinal Ulceration,” International Journal of Experimental Pathology, Vol. 84, No. 5, 2003, pp. 231-237. doi:10.1111/j.1365-2613.2003.00360.x
[17] B. D. Reid and B. K. Wilson, “Radiation processing technology for cosmetics,” Radiation Physics and Chemistry, Vol. 42, No. 4-6, 1993, pp. 595-596. doi:10.1016/0969-806X(93)90331-N
[18] G. P. Jacobs, “A review of the Effect of Gamma Irradiation on Pharmaceutical Materials,” Journal of Biomaterials Applications, Vol. 10, 1995, pp. 61-72.
[19] N. Barbarin, B. Tilquin, E. Dehoffmann, “Radiosterilization of Cefotaxime: Investigation of Potential Degradation Compounds by Liquid Chromatography Electrospray Mass Spectrometry,” Journal of Chromatography A, Vol. 929, No. 1-2, 2001, pp. 51-61. doi:10.1016/S0021-9673(01)01175-X
[20] N. Naki Sivri, A. Y. Ozer, M. Ozalp, N. Atakan and M. Polat, “Decontamination of Cosmetics Products and Raw Materials by Gamma Irradiation,” Journal of Pharmaceutical Sciences, Vol. 31, 2006, pp. 198-209.
[21] G. R. Dietz and W. J. Maher, “Radiation Sterilization in the Cosmetic Industry Update,” Cosmetics & Toiletries, Vol. 97, 1982, pp. 96-98.
[22] G. Prado, M. C. Andrade, M. S. Oliveira, A. S. Leal, B. R. Oliveira, L. R. Batista, “Efeito da IrradiaCAo na Microbiota Fúngica de Plantas Medicinais,” Ciência Agrotecnologia, Vol. 33, No. 5, 2009, pp. 1372-1378.
[23] L. C. Satomi, R. R. Soriani and T. J. A. Pinto, “Descontaminacao de Drogas Vegetais Empregando Irradiacao Gama e óxido de Etileno: Aspectos Microbianos e Químicos,” Revista Brasileira de Ciencias Farmaceuticas, Vol. 41, No. 4, 2005, pp. 445-450.
[24] R. S. Oliveira and W. Colaco, “Radiossensitividade Gama de Extrato de Maytenus ilicifolia: Desenvolvimento de Protocolo Para Controle de Qualidade,” Latin American Journal of Pharmacy, Vol. 27, No. 2, 2008, pp. 235-239.
[25] D. H. Bing, J. G. M. Weyand and A. B. Stavinsky, “Hemagglutination with Aldeyde-Fixed Erythrocytes for Assay of Antigens and Antibodies,” Proceedings of the Society for Experimental Biology and Medicine, Vol. 124, No. 4, 1967, pp. 1166-1170.
[26] O. H. Lowry, N. J. Rosebrough, A. L. Farr and R. J. Randall, “Protein Measurement with the Folin Phenol Reagent,” The Journal of Biological Chemistry, Vol. 193, No. 1, 1951, pp. 265-275.
[27] W. J. Maher and G. R. Dietz, “Radiation Sterilization in the Cosmetic Industry,” Cosmetics & Toiletries, Vol. 96, 1981, pp. 53-58.
[28] N. Roy, et al., “Permeability and Biocompatibility of Novel Medicated Hydrogel Wound Dressings,” Soft Materials, Vol. 8, No. 4, 2010, pp. 338-357. doi:10.1080/1539445X.2010.502955
[29] N. Roy, N. Saha, T. Kitano and P. Saha, “Novel Hydrogels of PVP-CMC and Their Swelling Effect on Viscoelastic Properties,” Journal of Applied Polymer Science, Vol. 117, No. 3, 2010, pp. 1703-1710.
[30] S. Choi and M. Chung, “A Review on the Relationship between Aloe Vera Components and Their Biologic Effects,” Seminars in Integrative Medicine, Vol. 1, No. 1, 2003, pp. 53-62. doi:10.1016/S1543-1150(03)00005-X
[31] G. C. Lopes, A. C. Sanches, C. V. Nakamura, B. P. Dias Filho, L. Hernandes and J. C. de Mello, “Influence of Extracts of Stryphnodendron polyphyllum Mart. and Stryphnodendron obovatum Benth. on the Cicatrisation of Cutaneous Wounds in Rats,” Journal of Ethnopharmacology, Vol. 99, No. 2, 2005, pp. 265-272. doi:10.1016/j.jep.2005.02.019
[32] C. M. L. Melo, E. R. C. Albuquerque, M. M. M. Oliveira, L. C. B. B. Coelho, M. C. O. C Coelho, M. T. S. Correia, et al., “Topical Use of Cratylia mollis Lectin in Wound Healing in Mice,” Reuniao Anual da Sociedade Brasileira de Bioquímica e Biologia Molecular, SBBq, Recife, 2003, p. 112.
[33] C. M. L. Melo, C. S. Porto, M. R. Melo-Júnior, C. M. Mendes, C. C. B. Cavalcanti, L. C. B. B. Coelho, et al., “Healing Activity Induced by Cramoll 1,4 Lectin in Healthy and Immunocompromised Mice,” International Journal of Pharmaceutics, Vol. 408, No. 1-2, 2011, pp. 113-119. doi:10.1016/j.ijpharm.2011.02.011

comments powered by Disqus

Copyright © 2020 by authors and Scientific Research Publishing Inc.

Creative Commons License

This work and the related PDF file are licensed under a Creative Commons Attribution 4.0 International License.