The CD133/1+ cell subset from human subcutaneous adult fat retains hemogenic potential


Research has shown that cells from adult fat tissue can effect long-term blood reconstitution. Fat-derived multipotentiality was ascribed to CD34+ perivascular populations from its prominent microvasculature, that represent mostly non-hemogenic, mesenchymal cells, although this tissue contains a CD34+45+ subset committed to a hemogenic fate. Here, in order to analyze cell subsets presenting hemogenic capabilities within fat, CD133/1+ and pericytes, the latter defined by CD140b (PDGFRb, Platelet-Derived Growth Factor Receptor Beta) expression, were immunomagnetically selected from stromal-vascular fractions (SVF). In Vitro Colony Forming Unit (CFU) assays were negative for CD140b+ pericytes and positive for CD133/1+ cells when a prolonged CFU assay was performed, revealing fat as another store of primitive progenitors that retain hemogenic potential.

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Santos de Moraes, C. , Roberto Albuquerque Leal, P. , Fabiano Ferreira, D. , Serra, F. , Abdelhay, E. and Sondermann Freitas, C. (2012) The CD133/1+ cell subset from human subcutaneous adult fat retains hemogenic potential. Stem Cell Discovery, 2, 36-40. doi: 10.4236/scd.2012.22006.

Conflicts of Interest

The authors declare no conflicts of interest.


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