Analytical and Stability Studies on Medical Cosmetics


Two simple and sensitive spectrofluorimetric (method Ι) and spectrophotometric (method ΙΙ) methods have been developed for the determination of some chloride containing toothpastes and panthenol-containing cosmetic preparations respectively. Method Ι is based on quantitative fluorescence quenching of (terbium-salicylate-hexamine ternary complex) by fluoride which could be measured at λem/λex of 547 nm/322 nm. The ΔF-concentration plot was rectilinear over the concentration range of 0.5 - 20 μg/ml. Method ΙΙ depends reaction of panthenol with nitrobenzoxadiazole chloride (NBD-Cl) and measuring the absorbance of the resultant product at 480 nm. The absorbance-concentration plot was rectilinear over the concentration range of 2 - 20 μg/ml.

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M. I. Eid and M. E. K. Wahba, "Analytical and Stability Studies on Medical Cosmetics," American Journal of Analytical Chemistry, Vol. 3 No. 3, 2012, pp. 277-281. doi: 10.4236/ajac.2012.33037.

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The authors declare no conflicts of interest.


[1] “The United States Pharmacopoeia 30, the National Formulary 25,” US Pharmacopeial Convention,” Rockville, 2007.
[2] W. Thies, “Direct Spectrophotometric Measurements on Sodium Citrate and Panthenol Solutions,” Pharmazie, Vol. 50, 1983, pp. 487-488.
[3] D. Bertini, V. Nuti and G. Linari, “Quantitative Determination of Compounds Present in a Pharmaceutical Preparation (Antiseborrhoeic shampoo),” Bollettino Chimico Farmaceutico, Vol. 121, 1982, pp. 535-544.
[4] M. Shehata, S. Tawakkol and L. A. Fattah, “Colorimetric and Fluorimetric Methods for Determination of Panthenol in Cosmetic and Pharmaceutical Preparations,” Journal of the Pharmaceutical Biomedical Analysis, Vol. 27, No. 5, 2002, pp. 729-735. doi:10.1016/S0731-7085(01)00510-6
[5] A. Takasu and K. Oyha, “Separation and Determination of the Enantiomers of Pantolactone by Gas-Liquid Chromatography,” Journal of Chromatography, Vol. 389, No. 1, 1987, pp. 251-255. doi:10.1016/S0021-9673(01)94430-9
[6] W. A. Koening and V. Sturm, “Determination of Optical Purity by Enantioselective Capillary Gas Chromatography; Panthenol and Relative Compounds,” Journal of Chromatography, Vol. 328, 1985, pp. 357-361. doi:10.1016/S0021-9673(01)87409-4
[7] V. S. Slyshenko and A. G. Moiseenok, “Gas Chromatographic Determination of Pantothenic Acid (Derivatives) in Vitamin Containing Prepara-tions,” Khimko Farmatsev- ticheskii, Vol. 17, 1983, pp. 1513-1516.
[8] K. D. Altria, “Background Theory and Application of Microemulsion Electrokinetic Chromatography,” Journal of Chromatography, Vol. 892, No. 1-2, 2000, pp. 171- 186. doi:10.1016/S0021-9673(00)00088-1
[9] R. K. Ghareh-bagh and S. Ebel, “Stability Analysis of Dexpanthenol. 1-Determination of Dexpanthenol and Pantolactone by HPLC,” Pharmazie, Vol. 50, No. 1, 1995, pp. 39-40.
[10] T. S. Hudson, S. N. Subramanian and R. J. Allen, “Determination of Panto-thenic Acid (and Its Alcoholic Analogue Dexpanthenol), Biotin, and Vitamin B12 (Cyanoco-balamine) in Nutritional Products,” Journal of the Association of Official Analytical Chemists, Vol. 67, 1984, pp. 994-998.
[11] S. S. Nag and S. K. Das, “Identification and Quantitation of Panthenol and Pantothenic Acid in Pharmaceutical Preparations by Thin Layer Chromatography and Densitometry,” Journal of AOAC International, Vol. 75, 1992, pp. 898-901.
[12] J. Karhan, Z. Ksander, Z. Srank and K. Koblas, “Determination of Panthenol and Tocopherol in Medicaments by NMR Spectroscopy,” Cesk Farm, Vol. 53, 1986, pp. 392-395.
[13] M. A. M. Shehata, M. A. Sultan, S. M. Tawakkol and L. E. A. Fatah, “Spec-trofluorimetric Method for Determina- tion of Panthenol in Cosmetic and Pharmaceutical Formulations,” Saudi Pharma-ceutical Journal, Vol. 12, 2004, pp. 29-34.
[14] O. A. Zapo-rozhets and L. Y. Tsyukalo, “Determination of Fluoride and Oxalate Using the Indicator Reaction of Zr(IV) with Methyl-thymol Blue Adsorbed on Silica Gel,” Analytica Chimica Acta, Vol. 597, No. 1, 2007, pp. 171- 177. doi:10.1016/j.aca.2007.06.029
[15] K. Shimada, T. Shimoda, H. Kokusen and S. Nakano, “Automatic Microdistillation Flow-Injection System for the Spectrophotometric Determination of Fluoride,” Talanta, Vol. 66, No. 1, 2005, pp. 80-85. doi:10.1016/j.talanta.2004.09.026
[16] D. G. Themelis, P. D. Tzanavaras and H. D. Tzanavaras, “Simple, Rapid Reagent-Injection Spectrophotometric Determination of Fluorides in Pharmaceutical Formulations,” Journal of Pharmaceutical Biomedical Analysis, Vol. 25, No. 5-6, 2001, pp. 971-976. doi:10.1016/S0731-7085(01)00373-9
[17] D. G. Themelis and P. D. Tzanavaras, “Simultaneous Spectrophotometric Determi-nation of Fluoride and Monofluorophosphate Ions in Tooth-pastes Using a Reversed Flow Injection Manifold,” Analytica Chimica Acta, Vol. 429, No. 1, 2001, pp. 111-116. doi:10.1016/S0003-2670(00)01271-X
[18] R. Lipka, M. Sobczak, K. Stanis, S. O. ?dowski and M. Jarosz, “Determina-tion of Fluoride Impurities in Leu-prolide. Comparison of Analytical Methods,” Microchemical Journal, Vol. 65, No. 1, 2000, pp. 51-58. doi:10.1016/S0026-265X(00)00028-X
[19] J. A. Nasr, Master’s Thesis, Mansoura University, Man- soura, 2005.
[20] G. H. Lu, Q. L. Wang, X. G. Wu, T. Zhan and X. Yao, “Polaro-graphic Determination of Trace Fluoride in Foods,” Food Chemistry, Vol. 66, No. 4, 1999, pp. 519-523. doi:10.1016/S0308-8146(99)00091-6

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