Antimicrobial Activity of Cannabis sativa L.


The oil of the seeds, petroleum ether and methanol extracts of the whole plant of Cannabis sativa belonging to the family Cannabinaceae were screened for their antimicrobial activity against two Gram positive organisms (Bacillus subtilis, Staphylococcus aureus), two Gram negative organisms (Escherichia coli, Pseudomonas aeruginosa) and two fungi namely Aspergillus niger and Candida albicans using the cup plate agar diffusion method. The oil of the seeds of Cannabis sativa exerted pronounced antibacterial activity (21 - 28 mm) against Bacillus subtilis and Staphylococcus aureus, moderate activity (15 mm) against Escherichia coli and high activity (16 mm) against Pseudomonas aeruginosa and inactive against the two fungi tested. The petroleum ether extract of the whole plant exhibited pronounced antibacterial activity (23 - 28 mm) against both Bacillus subtilis and Staphylococcus aureus organisms, high activity (16 mm) against Escherichia coli and inactive against Pseudomonas aeruginosa and both fungi. The methanol extract of the whole plant showed also pronounced antibacterial activity (29 mm) against Bacillus subtilis, low activity (12 mm) against Staphylococcus aureus and high activity (16 - 18 mm) against both Gram negative organisms, inactive against Aspergillus niger and low activity (13 mm) against Candida albicans. The minimum inhibitory concentrations of Cannabis sativa methanol extracts of the seeds and the whole plant against the standard organisms were determined using the agar plate dilution method. The standard organisms were tested against reference antibacterial and antifungal drugs and the results were compared with the activity of the extracts.

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E. Ali, A. Almagboul, S. Khogali and U. Gergeir, "Antimicrobial Activity of Cannabis sativa L.," Chinese Medicine, Vol. 3 No. 1, 2012, pp. 61-64. doi: 10.4236/cm.2012.31010.

Conflicts of Interest

The authors declare no conflicts of interest.


[1] N. Sachindra and A. Pradhan, “Marijuana Drug Abuse Clinical and Basic Aspects,” The C.V. Mosby Company, Saint Louis, 1977, pp. 148-173.
[2] D. M. Lambert and C. J. Fowler, “The End Cannabinoids System Drug Targets Lead Compounds and Potential Therapeutic Application,” Journal of Medicinal Chemistry, Vol. 48, No. 16, 2005, pp. 59-87.
[3] R. Mechoulam and N. Lander, “Cannabis a Possible Source of New Drugs,” Journal Pharmacy International, Vol. 1, 1980, pp. 19-21.
[4] B. Dilara and S. C. Nath, “Ethno Botanical Review of Medicinal Plants Used for Skin Diseases and Related Problems in Northeastern India,” Journal of Herbal Spices and Medicinal Plants, Vol. 7, No. 3, 2000, pp. 55-93. doi:10.1300/J044v07n03_07
[5] K. J. Kabelik, Z. Krejei and F. Santavy, “Cannabis as a Medicament,” Bulletin on Narcotic, Vol. 12, No. 3, 1960, pp. 5-23.
[6] E. Russo, “Cannabis Treatments in Obstetrics and Gynecology: Historical Review,” Journal of Cannabis Therapeutics, Vol. 2, No. 3-4, 2002, pp. 5-35.
[7] R. C. Clarke, “Filed Interview Schedule and Questionnaire for Investigating Cannabis Use, Rockville National Institute on Drug Abuse,” Journal Indian Hemp, Vol. 7, No. 1, 2002, pp. 83-88. doi:10.1300/J237v07n01_07
[8] K. Wasim, I. U. Haq and M. Ashraf, “Antimicrobial Studies of the Leaf of Cannabis sativa L.,” Pakistan Journal of Pharmaceutical Sciences, Vol. 8, 1995, pp. 22-38.
[9] J. Novak, K. Zitterl-Eglseer, S. G. Deans and C. M. Franz, “Essential Oils of Different Cultivars of Cannabis sativa L. and their Antimicrobial Activity,” Flavour Fragrance Journal, Vol.16, No. 4, 2001, pp. 259-262. doi:10.1002/ffj.993
[10] O. Polunin, “Flowers of Europe: A Field Guide,” Oxford University Press, London, 1969, pp. 56-57.
[11] D. Baker, G. Pryce, G. Giovannoni and A. J. Thompson, “The Therapeutic Potential of Cannabis,” Lancet Neurology, Vol. 2, No. 5, 2003, pp. 291-298. doi:10.1016/S1474-4422(03)00381-8
[12] J. L. Hartwell, “Plants Used against Cancer: A Survey 1967-1971,” Lloydia, 1971, pp. 30-34.
[13] S. Foster, “Herbs for Your Health,” Interwave Press, Loveland, 1996, pp. 56-57.
[14] C. Leizer, D. Ribnicky, A. Poulev, S. Dushenkov and I. Raskin, “The Composition of Hemp Seed Oil and Its Potential as an Important Source of Nutrition,” Journal of Nutraceuticals, Functional & Medical Foods, Vol. 2, No. 4, 2000, pp. 35-53. doi:10.1300/J133v02n04_04
[15] C. R. Langezaal, A. Chandra and J. J. C. Scheffer, “Antimicrobial Screening of Essential Oils and Extracts of Some Humulus lupulus L. Cultivars,” Pharmacy World & Science, Vol. 14, No. 6, 1992, pp. 353-356. doi:10.1007/BF01970171
[16] F. Kavanagh, “Analytical Microbiology,” Academic Press, New York and London, Vol. II, 1972.
[17] J. E. Blair, E. H. Lenette and I. P. Truant, “Manual of Clinical Microbiology. American Society for Microbiology,” Baltimore, Maryland and Wilkins Company, Washington DC, 1970.
[18] J. R. Borchardt, D. L. Wyse, C. C. Sheaffer, K. L. Kauppi, R. G. Fulcher, N. J. Ehlke, D. D. Biesboer and R. F. Bey, “Antimicrobial Activity of Native and Naturalized Plants of Minnesota and Wisconsin,” Journal of Medicinal Plants Research, Vol. 2, No. 5, 2008, pp. 98-110.

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