Prognostic factors correlation between the cell cycle phases and apoptosis in basal cell carcinoma
Cyro Festa-Neto, Durvanei Augusto Maria
DOI: 10.4236/ojim.2011.13019   PDF   HTML   XML   4,770 Downloads   8,046 Views   Citations


Basal cell carcinoma (BCC) is the most common skin malignancy, are found in various forms depending on their clinical and biological behavior. The objective of study was analyzed the phases of the cell cycle and correlations between BCC of low and high risk of recurrence and correlation prognostic factors. The quantity of content DNA in tissues of normal skin, showed small amount of cells in apoptosis and mostly in phase quiescent and rare aneuploidy cells. In BCC, apoptosis was higher in the BCC at high risk than low risk, probably due to their high rates of cell proliferation, and present of aneuploidy cells, when compared to the average percentage of aneuploidy. The DNA content from cells of normal skin shows that the majority is in the quiescent phase; compatible with tissues that are is refreshing. There is presence of apoptosis in the epidermis by probable normal process of differentiation. The aneuploidy in BCC showed a direct correlation with the degree of tumor aggressiveness.

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Festa-Neto, C. and Maria, D. (2011) Prognostic factors correlation between the cell cycle phases and apoptosis in basal cell carcinoma. Open Journal of Internal Medicine, 1, 99-104. doi: 10.4236/ojim.2011.13019.

Conflicts of Interest

The authors declare no conflicts of interest.


[1] Miller, S.J. (1995) Etiology and pathogenesis of basal cell carcinoma. Clinics in Dermatology, 13, 527-536. doi:10.1016/0738-081X(95)00062-K
[2] Dixon, A.Y., Lee, S.H. and MacGregor, D.H. (1989) Factors predictive of recurrence of basal cell carcinomas. American Journal of Dermatopathology, 11, 222-232. doi:10.1097/00000372-198906000-00005
[3] Pietras, K. and Ostman, A. (2010) Hallmarks of cancer: interactions with the tumor stroma. Experimental Cell Research, 316 ,1324-1331. doi:10.1016/j.yexcr.2010.02.045
[4] Cornelisse, C.J., Deville, P. and Smit, V.H. (1993) DNA Content as a genetic marker of cancer cells. In: Bauer, K.D., Duque, R.E. and Shankey, T.V., Eds., Clinical Flow Cytometry: Principles and Application, Williams & Wilkins; Baltimore, 3-12.
[5] Barlogie, B. Johnston, D.A., Smallwood, L. et al. (1982) Prognostic implications of ploidy and proliferative activity in human solid tumours. Cancer Genetics and Cytogenetics, 6, 17-28. doi:10.1016/0165-4608(82)90017-6
[6] Slater, D.N. (2004) Standards and minimum datasets for reporting cancers minimum dataset for the histopatho- logical reporting of common skin cancers. Royal College of Pathologists, London, 1-23.
[7] Duesberg, R., Li, P., Fabarius, A., Hehlmann, R. (2005) The chromosomal basis of cancer. Cellular Oncology, 27, 293-318.
[8] Kops, G.L., Weaver, B.A., Cleveland, D.W. (2005) On the road to cancer: Aneuploidy and the mitotic checkpoint. Nature Reviews Cancer, 5, 773-785. doi:10.1038/nrc1714
[9] Koutsami, M.K., Tsantoulis, P.K., Kouloukoussa, M., et al. (2006) Centrosome abnormalities are frequently observed in non-small-cell lung cancer and are associated with aneuploidy and cyclin E overexpression. Journal of Pathology, 209, 512-521. doi:10.1002/path.2005
[10] Frentz, G., Moller, U., Christensen I. (1980) DNA flow cytometry of human epidermis: I. methodological studies on normal skin. Journal of Investigative Dermatology, 74, 119-21. doi:10.1111/1523-1747.ep12535006
[11] Frentz, G. and Moller, U. (1981) DNA measurements by single nuclei flow cytometry in human actinic skin lesions. Journal of Investigative Dermatology, 77, 3133. doi:10.1111/1523-1747.ep12495660
[12] Frentz, G., Moller, U. and Keiding, N. (1982) DNA flow cytometry of human epidermis. The effect of serial biopsy sampling at various times. British Journal of Dermatology, 107, 7-14. doi:10.1111/j.1365-2133.1982.tb00283.x
[13] Frentz, G. and Moller, U. (1983) DNA flow cytometry of human epidermis: Interindividual and regional variations in normal skin. Clinical and Experimental Dermatology, 1, 19-26. doi:10.1111/j.1365-2230.1983.tb01739.x
[14] Gray, J.W., Carver, J.H., George, Y.S. and Mendelsohn, M.L. (1977) Rapid cell cycle analysis by measurement of the radioactivity per cell in narrow window in S phase. Cell and Tissue Kinetics, 10, 97-109.
[15] Weil, M., Raff, M.C. and Braga, V.M. (1999) Caspase activation in the terminal differentiation of human epidermal keratinocytes. Current Biology, 8, 361-364. doi:10.1016/S0960-9822(99)80162-6
[16] Frentz, G. and Moller, U. (1984) DNA flow cytometry of the epidermis of patients with multiple epidermal car- cinomas. British Journal of Dermatology, 111, 271-277. doi:10.1111/j.1365-2133.1984.tb04723.x
[17] Frentz, G., Moller, U. and Larsen, J.K. (1985) DNA flow cytometry of human epidermal tumours. Intra- and inter- tumour variability in ploidy and proliferative characteristics. Virchows Arch B Cell Pathol Incl Mol Pathol., 48, 175-183. doi:10.1007/BF02890126
[18] Buchner, T.H., Hiddemann, W. and Schumann, J. (1985) DNA aneuploidy a common cell marker in human malignancies and its correlation to grade, stage, and prognosis. In: Buchner, T.H., Bloomfield, C.D., Hiddemann, W., Hassfeld, D.K. and Schumann, J., Eds., Tumor Aneuploidy, Springer-Verlag, New York, 41-52.
[19] Friedlander, M.L., Hedley, D.W. and Taylor, I.W. (1984) Clinical and biological significance of aneuploidy in human tumours. Journal of Clinical Pathology, 37, 961- 974. doi:10.1136/jcp.37.9.961
[20] Koyama ,N., Nishihira, J., Nakabayashi, H., et al. (2000) Aneuploidy of sex chromosomes in basal cell carcinoma: its clonality and involvement in the development of carcinogenesis. International Journal of Oncology, 16, 5-23.
[21] Fortier-Beaulieu, M., Laquerrière, A., Thomine, E., Lauret, P. and Hémet, J. (1994) NA flow-cytometric analysis of basal cell carcinomas and its relevance to their morphological differentiation: A retrospective study. Dermatology, 188, 94-99. doi:10.1159/000247109
[22] Miller, S.J. (1991) Biology of basal cell carcinoma. Journal of the American Academy, 24, 1-13. doi:10.1016/0190-9622(91)70001-I
[23] Pappas, A.A., Maners, A.W., Owens, R.B., Thompson, J.R., King, J.C. and Dinehart, S.M. (1992) Deoxyribonucleic acid (DNA) ploidy and proliferative characteristics of metastatic squamous cell carcinoma determined by flow cytometric analysis. Journal of Dermatologic Surgery and Oncology, 18, 957-960.
[24] Robinson, J.K., Rademaker, A.W., Goolsby, C., Traczyk, T.N. and Zoladz, C. (1996) DNA ploidy in nonmelanoma skin cancer. Cancer, 77, 284-291. doi:10.1002/(SICI)1097-0142(19960115)77:2<284::AID-CNCR10>3.0.CO;2-Y
[25] Bolshakov, S., Walker, C.M., Strom, S.S., et al. (2003) p53 mutations in human aggressive and nonagressive ba- sal and squamous cell carcinomas. Clinical Cancer Research, 9, 228-234.
[26] Cerroni, L. and Kerl, H. (1994) Aberrant bcl-2 protein expression provides a possible mechanism of neoplastic cell growth in cutaneous basal-cell carcinoma. Cutaneous Pathology, 21, 398-403. doi:10.1111/j.1600-0560.1994.tb00279.x
[27] Delehedde, M., Cho, S.H., Sarkiss, M., et al. (1999) Altered expression of bcl-2 family member proteins in non- melanoma skin cancer. Cancer, 85, 1514-1522.
[28] Staibano, S., Lo Muzio, L., Pannone, G., et al. (2001) Interaction between bcl-2 and p53 in neoplastic progression of basal cell carcinoma of the head and neck. Anticancer Research, 21, 3757-3764.
[29] Wikonkal, N.M., Berg, R.J.W., van Haselen, C.W., et al. (1997) Bcl-2 versus p53 protein expression and apoptotic rate in human nonmelanoma skin cancer. Archives of Dermatology, 133, 599-602. doi:10.1001/archderm.1997.03890410055007
[30] Tille, C.M.L.J., Stavast-Kooy, A.J.W., Remaekers, F.C.S. and Neumann, H.A. (2002) Bax expression and growth behaviour of basal cell carcinomas. Journal of Cutaneous Pathology, 29, 79-87. doi:10.1034/j.1600-0560.2002.290203.x
[31] Staibano, S., Muzio, L.L., Mezza, E., Argenziano, G., Tornillo, L., Pannone, G. and De Rosa, G.(1999) Prognostic value of apoptotic index in cutaneous basal cell carcinomas of head and neck. Oral Oncology, 35, 541- 547. doi:10.1016/S1368-8375(99)00028-7

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