Restriction fragment length polymorphism in the exon 2 of the BoLA-DRB3 gene in Chinese Holstein of the south China

Xiu-Xiang Wu, Zhang-Ping Yang, Xiao-Long Wang, Yong-Jiang Mao, Shu-Chun Li, Xue-Kui Shi, Ying Chen

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DOI: 10.4236/jbise.2010.32030                

Abstract

The genetic diversity of the exon2 of BoLA-DRB3 (BoLA-DRB3.2) in Chinese Holstein cattle of the south China was investigated by hemi-nested polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technique. Six, four and eleven RFLP patterns were found after digestion with the restriction enzymes Hae III, Bst YI and Rsa I, respectively. The DNA sequence showed and twenty- five DRB3.2 alleles. GLM model analysis indicated that lactation and calving season have positive correlation with SCC (Somatic Cell Count) (p<0.01), BoLA-DRB3.2*3, *8, *18 and *26 were associated with lower SCC (p<0.01). The present findings concluded that mastitis is a severe hinder of milk production and technology. Therefore, future re-searches should focus on associations of mastitis with BoLA haplotypes rather than single BoLA genes

Keywords

PCR-RFLP; BoLA-DRB3.2; Chinese Holstein; Genetic Polymorphism; SCC

Conflicts of Interest

The authors declare no conflicts of interest.

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