Author(s)

Karoline Rios, Christian Vélez, Beatriz Zayas^*

School of Science and Technology and Environment, Universidad Ana G. Méndez, San Juan, Puerto Rico.

Phthalates have been used in a wide variety of consumer goods. Their versatility as plasticizers has translated into worldwide use in a vast array of consumer products. These compounds can leach into matrices, such as food and liquids that can be routed for human exposure. One of the most used phthalates is Diethylhexyl phthalate (DEHP). Diethylhexyl phthalate and its metabolite 2-ethyl-1-hexanol (2-EH) have demonstrated biological effects which merit further evaluation. In this work, we expand on our previous work with DEHP and screen the 2-EH metabolite for different cell death endpoints such as growth inhibition, apoptosis, autophagy, caspase activation, DNA fragmentation, and cell cycle arrest using fluorophores and the NC3000 instrument. Significant results (p < 0.05) revealed higher toxicity for the 2-EH metabolite when compared to DEHP. Also, 2-EH presented apoptosis induction with characteristic hallmarks, such as loss of mitochondrial membrane potential, caspase activation, DNA fragmentation and cell cycle arrest at the S phase. In addition, the presence of autophagosome was detected through L3CB protein staining. We conclude that 2-EH presents differences in cell death endpoints that interestingly differ from the DEHP parent compound. Further studies are needed to establish the molecular pathways responsible for the observed effects.

Phthalates, 2-Ethyl-1-Hexanol, Diethylhexyl Phthalate, Autophagy, Apoptosis, Lymphoblast, Caspase

Rios, K. , Vélez, C. and Zayas, B. (2019) Cell Death Effects of the Phthalate 2-Ethyl-1-Hexanol on Human Linfoblast Cells. Open Journal of Apoptosis, 8, 1-15. doi: 10.4236/ojapo.2019.81001.