The After cancer resection including bone, recently, bone resected with cancer has been considered to be reusable. We newly examined superheated steam treatment for bone reuse and compared it to existing treatments. Forty male C3H/HeN mice were used to establish a model of mandible invasion by oral squamous cell carcinoma (OSCC). The mice were sacrificed to harvest the tumor invading mandible bone. The resected tumor with mandible bone was treated by one of four kinds of treatment, no treatment for the control, the Pasteur method, liquid nitrogen treatment, micro-wave treatment and superheated steam treatment. After each treatment, the resected bone was transplanted into a syn-geneic mouse back. Eight weeks after transplantation, the mice were sacrificed and evaluated pathologically. Grafted tumors showed recurrence: 7/7 in the control, 6/8 in the liquid nitrogen treatment, 1/8 the microwave treatment and 2/8 the superheated steam treatment groups. No recurrence, on the other hand, was observed in the Pasteur method (0/8). The Pasteur method is a good treatment to remove malignant cells.