Author(s)

Jiming Li^1*, Meide Wei^2*, Lili Wang¹, Yanbo Wu¹, Lu Kang¹, Chunchun Su¹, Fengli Yang^3#, Guosheng Su^4,5#

¹Laboratory Department of the Second Affiliated Hospital of Guilin Medical University, Guilin, China.
²Laboratory Department of the Fourth Affiliated Hospital of Guangxi Medical University, Liuzhou, China.
³Department of Pediatrics, Nanning Third People’s Hospital, Nanning, China.
⁴Laboratory Department of People’s Hospital of Guangxi ASEAN Economic and Technological Development Zone, Nanning, China.
⁵Laboratory Department of Nanning 10th People’s Hospital, Nanning, China.

Abstract Objective: To explore the antibacterial activity of combined use of Shuanghuanglian and cefoperazone sulbactam sodium on resistant strains of Pseudomonas aeruginosa. Methods: The Pseudomonas aeruginosa strains which were sensitive and resistant to cefoperazone sulbactam sodium were selected to prepare different test bacterial solutions respectively; The experimental liquid of Shuanghuanglian and Cefoperazone Sulbactam Sodium were prepared separately and set as different test groups and control groups; The Drug Sensitivity Tests of Shuanghuanglian and cefoperazone sulbactam sodium at different concentration gradients which were used alone or used in combination were carried out for different strains with sensitivity and resistance, And use standard entry as a reference control. Result: The results of drug sensitivity test of Shuanghuanglian combined with Cefoperazone-Sulbactam sodium against the resistant strains of Pseudomonas aeruginosa were compared with the results of drug sensitivity test of the two separately used, and the difference was statistically significant (P < 0.05) [The drug sensitivity test results of Shuanghuanglian and cefoperazone sulbactam sodium to Pseudomonas aeruginosa resistant strains were statistically significant compared with the drug sensitivity test results of Shuanghuanglian and Cefoperazone Sulbactam Sodium used separately (P < 0.05)]; There was a dependence between strains and concentration in the effect of the combination of the two drugs. Conclusion: The combination of Shuanghuanglian and cefoperazone sulbactam sodium has synergistic antibacterial or bactericidal effect on Pseudomonas aeruginosa resistant strains.

Shuanghuanglian, Cefoperazone-Sulbactam Sodium, Use in Combination, Pseudomonas aeruginosa, Drug Resistance, Bacteriostasis

Li, J. , Wei, M. , Wang, L. , Wu, Y. , Kang, L. , Su, C. , Yang, F. and Su, G. (2023) Study on the Effect of Shuanghuanglian Powder Needle Combined with Cefoperazone and Sulbactam Sodium on Pseudomonas aeruginosa in Vitro. Advances in Microbiology, 13, 223-236. doi: 10.4236/aim.2023.135014.