Author(s)

Muhammad Danish Mehmood^1*, Huma Anwarul-Haq¹, Faisal Amin², Sajjad Hussain³, Ejaz Rafique⁴, Muhammad Usman Ghani¹, Muhammad Ismail¹, Fareeha Ghaffar⁴

¹Ottoman Pharma (Immuno Division), Lahore, Pakistan.
²Grandparent Diagnostic Laboratory, Lahore, Pakistan.
³Veterinary Research Institute, Lahore, Pakistan.
⁴Institute of Molecular Biology and Biotechnology, University of Lahore, Lahore, Pakistan.

Hydro-Pericardium Syndrome (HPS) is viral problem of commercial poultry caused by aviadeno virus type-4. In Pakistan the problems have been controlled by administering inactivated infected liver homogenate vaccine (ILHV). The use of liver based HPS vaccines remained potential threat for having hypersensitivity reactions in poultry. The current study was carried out to compare the serological potency of HPS ILHV to vero cell line adopted vaccine in term of anti HPS-ELISA antibody titers. 14 HPS virus vaccines were prepared based on different concentration of antigen, type of adjuvants and source of virus substrate. Total of 160 birds were divided into 16 groups each containing 10 birds. At day of 14^th age each bird of every group was injected with 0.3 ml dose of respective vaccine. It was observed that HPS infected liver based vaccine having 1 × 10^5.6, 1 × 10^5.6 and 1 × 10^3.6 bird lethal dose 50 induced 1092.10, 875.25 and 702.2 anti-HPS ELISA antibody titer respectively. The 20, 25 and 30 doses/gm HPS infected liver vaccine induced 110.4, 1071.9 and 1037.8 anti-HPS ELISA antibody titer respectively. Montanide based tissue culture HPS vaccine showed significantly higher 1148.45 anti-HPS ELISA antibody titer to aluminium hydroxide based vaccine (137.2) (P < 0.05). It is concluded that montanide based HPS vaccine prepared from tissue culture technique having biological titer ≥ 1 × 10^5.6 TCID₅₀ is serological potent against field infection. The vaccines based on such formulation could be prepared in future for effective immuno-prophylaxis against HPS virus.

Hydro-Pericardium Syndrome, Vero Cell Line, Infected Liver Homogenate, Cytopathic Effect, Tissue Culture Infective Dose, Montanide, HPS ELISA

Mehmood, M. , Anwarul-Haq, H. , Amin, F. , Hussain, S. , Rafique, E. , Ghani, M. , Ismail, M. and Ghaffar, F. (2020) Comparative Efficacy of Different Inactivated Hydro-Pericardium Syndrome Vaccines Prepared from Infected Liver and Vero Cell Line Adapted Adeno Type 4 Virus. World Journal of Vaccines, 10, 1-16. doi: 10.4236/wjv.2020.101001.