Author(s)

Aqsa Yousaf^1*, Ayesha Zafar¹, Muhammad Ali^2*, Snober Mona Bukhary³, Yasmeen Manzoor¹, Tuba Tariq¹, Asma Saeed², Muhammad Akram¹, Faryal Bukhari¹, Muhammad Abdullah⁴, Syeda Sadaf Zehra⁵, Shahbaz Gul Hassan⁶, Murtaza Hasan¹

¹Department of Biochemistry and Biotechnology, The Islamia University, Bahawalpur, Pakistan.
²Department of Biological Sciences, Gomal University, Dera Ismail Khan, Pakistan.
³Department of Chemistry, The Islamia University, Bahawalpur, Pakistan.
⁴Cholistan Institute of Desert Studies (CIDS), The Islamia University, Bahawalpur, Pakistan.
⁵Department of Botany, The Islamia University, Bahawalpur, Pakistan.
⁶College of Information Science and Engineering, Zhongkai University of Agriculture and Engineering, Guangzhou, China.

Objective: Evaluating the bio-reducing activity and various potential, annotates the biological entitties of Fagonia cretica. Method: By fractionating the crude extract with different ethanol-water fraction mixture. This substantiates the reduction potential for the biological synthesis of AgNPs, its antioxidant, anti-urease and anti-Tyrosinase activity. Results: UV-visible spectra confirm the bio-reducing potential for Ag NPs with the optimized 50% ethanol-water fraction mixture. Transmission electron microscopy (TEM) confirmed the round shaped AgNPs with an average size of 16 nm in optimized 50% fraction mixture. Further evaluating Ethanol-water fraction with 90% and 70% of plant extract showed more potentials for Anti-Tyrosinase and Anti-urease activity. Moreover, diluted 30% fraction exhibits two folds higher Anti-oxidant and Anti urease activity. Conclusion: Overall, this work showed that 50% Ethanol-water fraction has potentially active molecules (among all fractions) primarily involved in bio-reducing potential of Ag NPs and enzymatic assay while 90% mixture proved to be least active. This study suggested electing the optimum mixture fraction of 50% ethanol-water in order to screen the bioactive compounds and to inculcate their activity status for clinical trials.

Bioactivity, Fagonia cretica, Nanoparticles, Enzymes Assay, Bio Reducing Agent

Yousaf, A. , Zafar, A. , Ali, M. , Bukhary, S. , Manzoor, Y. , Tariq, T. , Saeed, A. , Akram, M. , Bukhari, F. , Abdullah, M. , Zehra, S. , Hassan, S. and Hasan, M. (2019) Intrinsic Bio-Enhancer Entities of Fagonia cretica for Synthesis of Silver Nanoparticles Involves Anti-Urease, Anti-Oxidant and Anti-Tyosinase Activity. Advances in Bioscience and Biotechnology, 10, 455-468. doi: 10.4236/abb.2019.1012032.