Advances in Microbiology

Volume 9, Issue 10 (October 2019)

ISSN Print: 2165-3402   ISSN Online: 2165-3410

Google-based Impact Factor: 1.28  Citations  h5-index & Ranking

Composition and Molecular Identification of Bacterial Community in Seawater Desalination Plants

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DOI: 10.4236/aim.2019.910053    295 Downloads   508 Views  

ABSTRACT

Biofouling is an important problem for reverse osmosis (RO) membrane manufacturers. Bacteria are mainly involved in generating fouling and obturating RO membranes. Insights into biofilm bacteria composition could help prevent biofouling, reduce the cost of using RO-fouling membranes and guarantee safe water. Culture-dependent and independent techniques were then performed in order to identify bacteria associated with RO membranes. Bacteria cultures described the presence of six pure colonies, four of which were identified through API testing. Based on 16s rRNA gene analysis, a predominant bacterium was identified and annotated as Sphingomonas sp. The 16s rRNA gene clone library, on the other hand, showed that the bacterium, Pseudomonas marincola, accounted for nearly 30% of the clone library, while the rest of bacteria were chimeras (62%) and non-representative species (3%). In conclusion, culture-dependent and independent approaches showed that two dominant bacteria were commonly observed in RO desalination membranes.

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Garcia-Jimenez, P. , Carrasco-Acosta, M. , Payá, C. , López, I. , Rodríguez, J. and Melián, J. (2019) Composition and Molecular Identification of Bacterial Community in Seawater Desalination Plants. Advances in Microbiology, 9, 863-876. doi: 10.4236/aim.2019.910053.

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