Fatty Acid Profiling of Polyethylene Glycol Adapted and Un-Adapted Cell Lines of Oryza sativa L.cv. Swat-1 under Temperature Stress ()
Affiliation(s)
1Institute of Crop Science, National Engineering Laboratory for Crop Molecular Breeding, Chinese Academy of Agricultural Sciences, Beijing, China.
2National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan, China.
3Institute of Biotechnology and Genetic Engineering, University of Agriculture, Peshawar, Pakistan.
ABSTRACT
Higher plants can adapt to abiotic stress to a certain degree. In this study, the impact of temperature stress on osmotic stress adapted and un-adapted cell lines of rice (Oryza sativa L.cv Swat-1) was observed. For the change in proline content, relative growth rate, saturated and unsaturated fatty acid were evaluated. The cell lines were incrementally adapted to 20% polyethylene glycol. The adapted lines showed significantly higher growth rate and proline content as compared to the un-adapted cell lines on temperature stress. Among saturated fatty acids palmitic acid (C16:0), stearic acid (C18:0) and myristic acid (C14:0) were the prominent fatty acids detected while among unsaturated fatty acid Oleic acid (C18:1c) and Linoleic acid (C18:2c) were the major fatty acids found. Under low temperature stress the percentage of saturated fatty acids was found to be lower (53%) in adapted cell line as compared to the un-adapted cell line (63%) while the percentage of saturation increased (83%) in adapted line under high temperature stress as compared to un-adapted line (70%). On the other hand at low temperature stress the percent level of unsaturated fatty acids in the adapted line was higher (48%) than the un-adapted cell line (37%). In conclusion, adaptation to one abiotic stress confers co-tolerance to the other abiotic stresses. Fatty acids saturation level could be a crucial factor in the plant ability to tolerate heat and cold stress.
Share and Cite:
Shah, S. , Ullah, F. and Shah, S. (2019) Fatty Acid Profiling of Polyethylene Glycol Adapted and Un-Adapted Cell Lines of
Oryza sativa L.cv. Swat-1 under Temperature Stress.
American Journal of Molecular Biology,
9, 145-153. doi:
10.4236/ajmb.2019.94011.
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