Investigation of DNAmethylation of TWIST Gene in Breast Cancer and Its Relationship to Histopathological Features ()
Affiliation(s)
1Department of Molecular Biology and Genetics, Faculty of Science and Letters, Bilecik Seyh Edebali University, Bilecik, Turkey.
2Biotechnology Research and Application Center, Bilecik Seyh Edebali University, Bilecik, Turkey.
3Department of Medical Genetics, Faculty of Medicine, Osmangazi University, Eskisehir, Turkey.
ABSTRACT
BACKGROUND: Promoter hypermethylation and global hypomethylation
in the human genome are hallmarks of most cancers. OBJECTIVE: The aim of this study is to assess the methylation
profile patterns of TWIST gene and to investigate the relationship of
methylation with pathological features. METHODS: Romoter CpGisland methylation of TWIST which can
be related in breast cancer was performed
by methylation-sensitive high resolution melting (MS-HRM) analysis.
Formalin-fixed, paraffin-embedded (FFPE) tissue samples of 80 patients with a
diagnosis of primary breast cancer from Eskisehir Osmangazi University medical
faculty of Oncology Clinic were included. RESULTS: In our study, the promoter hypermethylation
frequency of TWIST gene was 25.0%. With these results, when the prognostic factors
of the patients were analyzed, tumor stage and age were found to be meaningless
with the hypermethylation of TWIST gene, but found to be significant
with lymph node positivity, ER positivity,
PR negativity, and HER2/NEU negativity. CONCLUSION: Our study is important as being
the first study that analyzes association between histopathologic type and
TWIST gene promotor methylation status in Turkish population.
Share and Cite:
Eroglu, O. , Cilingir, O. , Artan, S. and Aras, B. (2019) Investigation of DNAmethylation of TWIST Gene in Breast Cancer and Its Relationship to Histopathological Features.
Advances in Breast Cancer Research,
8, 45-59. doi:
10.4236/abcr.2019.81004.