Advances in Reproductive Sciences

Volume 6, Issue 2 (May 2018)

ISSN Print: 2330-0744   ISSN Online: 2330-0752

Google-based Impact Factor: 0.86  Citations  

The Influence of Advanced Paternal Age on Sperm Chromatin Integrity and Early Embryo Morphological Development during ICSI

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DOI: 10.4236/arsci.2018.62004    817 Downloads   1,676 Views  Citations

ABSTRACT

The study determined the impact of advanced male ageing (≥50 years) on sperm chromatin integrity and early embryo morphological development in intra-cytoplasmic sperm injection (ICSI) technique cycles. Six hundred subfertile men were age-grouped; X1 (50 to 59 years), X2 (60 to 69), and X3 (≥70), were compared with 600 fertile males of known fertility (Y, age 25 - 35 years). Oocytes from 254 women, aged ≤ 30 years, were included. Sperm were analyzed using acridine orange fluorescence test (AOT) and categorized: “low”, “inter-mediate” and “high” damage. After ICSI, embryos were evaluated and categorized as “good”, “fair” or “poor” quality. Embryonic morphological development was assessed at three stages: fertilization, early and late paternal effect. The AOT results were: X1: low = 29, intermediate = 53 and high = 118; X2: low = 11, intermediate = 42 and high = 147; X3: low = 8, intermediate = 24 and high = 168; Y: Low = 486, intermediate = 71 and high = 43. The fertilization rate was: X1, 329/350 (93.7%); X2, 298/350 (85.1%); X3, 225/350 (64.1%) and, Y, 350/350 (100%). Associations between increasing age and sperm chromatin damage (χ2 (723.249, 6) p < 0.0001), increasing age and inability to fertilize (χ2 (210.990, 3) p < 0.0001) were observed. Associated with increasing age was the significant proportion of morphologically poor quality embryos over the five days after fertilization. Male age ≥ 50 years, is highly associated with abnormal sperm chromatin organization, an inability to adequately fertilize with ICSI methodology, an increase in the number of poor quality embryos and, a corresponding decrease in the number of good quality embryos five days after fertilization.

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Dickson, M. , M. Adedia, D. and A. Abaye, D. (2018) The Influence of Advanced Paternal Age on Sperm Chromatin Integrity and Early Embryo Morphological Development during ICSI. Advances in Reproductive Sciences, 6, 35-49. doi: 10.4236/arsci.2018.62004.

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