Antimutagenic and DNA Damage Protective Activities of a Grape Extract from Vitis vinifera - Journal of Biosciences and Medicines

JBM > Vol.4 No.6, June 2016

Journal of Biosciences and Medicines

Volume 4, Issue 6 (June 2016)

ISSN Print: 2327-5081 ISSN Online: 2327-509X

Google-based Impact Factor: 0.51 Citations

Antimutagenic and DNA Damage Protective Activities of a Grape Extract from Vitis vinifera ()

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DOI: 10.4236/jbm.2016.46001 2,301 Downloads 3,337 Views Citations

Author(s)

Zuzana Rybková^*, Kateřina Malachová, Jiří Červeň, Hana Sezimová, Petr Pečinka

Affiliation(s)

Institute of Environmental Technologies, Department of Biology and Ecology, Faculty of Science, University of Ostrava, Ostrava, Czech Republic.

ABSTRACT

Antimutagenic and DNA protective effect of an extract VinOserae from Vitis vinifera grapes on oxidative DNA damage was investigated. The extract’s ability to inhibit mutagenicity induced by tert-butyl hydroperoxide (t-BHP) and hydrogen peroxide (H2O2) was determined with Ames test using Salmonella typhimurium His? TA102 strain. Inhibition values of 44.2% and 67.0% were detected for t-BHP and H2O2, respectively. A protective ability of the extract against DNA strand scission induced by hydroxyl radicals was studied with plasmid pBluescript II SK(-). The analysis of DNA strand breaks in plasmid DNA showed a significant inhibition of DNA damage.

KEYWORDS

Ames Test, Antimutagenicity, Free Radical Scavenging, DNA Strand Scission, Vitis vinifera

Share and Cite:

Rybková, Z. , Malachová, K. , Červeň, J. , Sezimová, H. and Pečinka, P. (2016) Antimutagenic and DNA Damage Protective Activities of a Grape Extract from Vitis vinifera. Journal of Biosciences and Medicines, 4, 1-8. doi: 10.4236/jbm.2016.46001.

Cited by

[1]	Protective effect of leaf extract of Abutilon indicum on DNA damage and peripheral blood lymphocytes in combating the oxidative stress
	2020

[2]	Analysis of antioxidant and antigenotoxic activities of grape berry extracts
	2018

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