Author(s)

Satoshi Fukushima¹, Keiichi Motoyama², Yuki Tanida², Taishi Higashi², Yoichi Ishitsuka², Yuki Kondo², Tetsumi Irie^2,3, Takumi Tanaka⁴, Hironobu Ihn^1*, Hidetoshi Arima^2,3*

¹Department of Dermatology and Plastic Surgery, Faculty of Life Sciences, Kumamoto University, Kumamoto, Japan.
²Graduate School of Pharmaceutical Sciences, Kumamoto University, Kumamoto, Japan.
³Program for Leading Graduate Schools “HIGO (Health Life Science: Interdisciplinary and Glocal Oriented) Program”, Kumamoto University, Kumamoto, Japan.
⁴Daito Kasei Kogyo Co., Ltd., Osaka, Japan.

Atopic dermatitis is a skin disease characterized by inflammation, pruritus, and chronic or relapsing eczematous lesions. Recently, ampholytic polysaccharide sacran has attracted a particular focus of attention as a novel biomaterial. In the present study, we investigated the effect of sacran solution on atopic dermatitis in the clinical study. Almost all of the average scores for atopic dermatitis symptoms of each patient treated with sacran solutions were improved. In addition, the scores of sleep disorder and itching were also significantly ameliorated by the sacran treatment for 4 weeks, compared with those of initial states. In immatured dermal skin model stimulated with 2,4-dinitrofluorobenzene (DNFB), the sacran treatment markedly down-regulated inflammatory cytokine and chemokine mRNA levels such as MCP-1, TNF-α, IL-1β, and IL-6 mRNAs, compared with that of DNFB alone. Furthermore, a sacran solution significantly suppressed the mRNA expression of TNF-α and COX-2 in RAW264.7 cells, a murine macrophage-like cell line, stimulated with phorbol-12-myristate-13-acetate (PMA). In RBL-2H3 cells, a rat basophilic leukemia cell line, a sacran solution significantly lowered β-hexosaminidase release, indicating the suppression of allergic response. These results suggest that a sacran solution may have the potential to improve atopic dermatitis through the impairment of production of inflammatory cytokine and chemokine mRNA.

Sacran, Polysaccharide, Clinical Trial, Atopic Dermatitis, Skin Care

Fukushima, S. , Motoyama, K. , Tanida, Y. , Higashi, T. , Ishitsuka, Y. , Kondo, Y. , Irie, T. , Tanaka, T. , Ihn, H. and Arima, H. (2016) Clinical Evaluation of Novel Natural Polysaccharides Sacran as a Skincare Material for Atopic Dermatitis Patients. Journal of Cosmetics, Dermatological Sciences and Applications, 6, 9-18. doi: 10.4236/jcdsa.2016.61002.