Author(s)

Jinping Wu¹, Chaozhu Yang², Zhenbiao Jiao¹, Zhongli Hu³, Zili Ding^1*, Zhengming Qiu¹

¹Hubei Academy of Agricultural Sciences, Wuhan, China.
²Enshi Autonomous Prefecture Academy of Agricultural Sciences, Enshi, China.
³College of Life Sciences, Wuhan University, Wuhan, China.

Twenty-three isolates of soft rot bacteria from konjac corms were examined for their diversity using 16S rDNAs and AFLP technology. Both methods clustered two groups, dependent on their biotype characterization of Pectobacterium carotovora subsp. carotovora (P.c.c) and Pectobacterium chrysanthemi (P.ch), respectively. Of all isolates, 17 (73.9%) belonged to P. ch, indicated as the main pathogenic bacteria of konjac producing areas in China. The genetic variation among isolates from the same biotype was also rich, not consistent with the distances of the geographic sources.

Soft Rot Bacteria, Identification, 16S rDNA, AFLP, Genetic Diversity

Wu, J. , Yang, C. , Jiao, Z. , Hu, Z. , Ding, Z. and Qiu, Z. (2015) Genetic Relationships of Soft Rot Bacteria Isolated from Konjac in China by Amplified Fragment Length Polymorphism (AFLP) and 16S rDNA Gene Sequences. Agricultural Sciences, 6, 717-723. doi: 10.4236/as.2015.67069.