Open Journal of Urology

Volume 5, Issue 6 (June 2015)

ISSN Print: 2160-5440   ISSN Online: 2160-5629

Google-based Impact Factor: 0.20  Citations  h5-index & Ranking

Diagnostic and Analysis of Human Sperm Characteristics Using Fourier Transform Infrared Spectroscopy

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DOI: 10.4236/oju.2015.56015    3,279 Downloads   4,223 Views  Citations

ABSTRACT

A spectroscopic method for human sperm evaluation and characterization using Fourier Transform Infra Red (FTIR) is presented. The high sensitivity of FTIR to changes in chemical structure and arrangement of molecules and proteins makes it a powerful diagnostic tool. Our experimental results show that a simple MIR (400 cm-1 - 4000 cm-1) transmission spectrum of a human sperm is very fast and can be used to determine the level of structure, compare to conventional LAB tests. No sample preparations are required, the semen has to be put on a special ZnSe substrate and inserted into the measurement compartment of the FTIR. Furthermore, this method can distinguish between immature sperm cell to white blood cell which by using a microscope is difficult and re-quires experience.

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Abramovich, A. and Shulzinger, A. (2015) Diagnostic and Analysis of Human Sperm Characteristics Using Fourier Transform Infrared Spectroscopy. Open Journal of Urology, 5, 97-101. doi: 10.4236/oju.2015.56015.

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