A Polymerase Chain Reaction Based DNA Fingerprinting Technique: Amplified Fragment Length Polymorphism for Molecular Typing of Perchlorate Reducing Bacteria ()
ABSTRACT
Genetic profiling
of environmentally important organisms is very essential for easy identification
of biodegrading bacteria. In the previous study, we have reported the
perchlorate biodegrading bacteria and characterized them by biochemical
analysis and 16 S sequencing. We have observed a very similar isolates of
Arthrobacter (Actinobacteria) degrading 4.1 mM and 4.7 mM of ammonium
perchlorate [1]. In this study, we report PCR based DNA fingerprinting technique to
generate the genomic signature of these closely related group of Arthrobacter
species. This study also effectively generates unique genomic signature for
each of these isolates that has potential for use in molecular monitoring as
well as for tracking genomic variation and rearrangements.
Share and Cite:
Shete, A. , Ghole, V. , Raina, M. and Aich, B. (2014) A Polymerase Chain Reaction Based DNA Fingerprinting Technique: Amplified Fragment Length Polymorphism for Molecular Typing of Perchlorate Reducing Bacteria.
Journal of Environmental Protection,
5, 1111-1115. doi:
10.4236/jep.2014.512109.
Cited by
No relevant information.