Journal of Agricultural Chemistry and Environment

Volume 3, Issue 2 (May 2014)

ISSN Print: 2325-7458   ISSN Online: 2325-744X

Google-based Impact Factor: 0.82  Citations  

Monitoring MVOC Profiles over Time from Isolates of Aspergillus flavus Using SPME GC-MS

HTML  XML Download Download as PDF (Size: 1733KB)  PP. 48-63  
DOI: 10.4236/jacen.2014.32007    4,374 Downloads   6,824 Views  Citations

ABSTRACT

Fungi produce a variety of microbial volatile organic compounds (MVOCs) during primary and secondary metabolism. The fungus, Aspergillus flavus, is a human, animal and plant pathogen which produces aflatoxin, one of the most carcinogenic substances known. In this study, MVOCs were analyzed using solid phase microextraction (SPME) combined with GCMS from two genetically different A. flavus strains, an aflatoxigenic strain, NRRL 3357, and a non-aflatoxigenic strain, NRRL 21882. A PDMS/CAR SPME fiber was used over 30 days to observe variations in MVOCs over time. The relative percentage of individual chemicals in several chemical classes (alcohols, aldehydes, esters, furans, hydrocarbons, ketones, and organic acids) was shown to change considerably during the varied fungal growth stages. This changing chemical profile reduces the likelihood of finding a single chemical that can be used consistently as a biomarker for fungal strain identification. In our study, discriminant analysis techniques were successfully conducted using all identified and quantified MVOCs enabling discrimination of the two A. flavus strains over the entire 30-day period. This study underscores the potential of using SPME GCMS coupled with multivariate analysis for fungi strain identification.

Share and Cite:

Sun, D. , Wood-Jones, A. , Wang, W. , Vanlangenberg, C. , Jones, D. , Gower, J. , Simmons, P. , Baird, R. and Mlsna, T. (2014) Monitoring MVOC Profiles over Time from Isolates of Aspergillus flavus Using SPME GC-MS. Journal of Agricultural Chemistry and Environment, 3, 48-63. doi: 10.4236/jacen.2014.32007.

Cited by

[1] Impact of Volatile Organic Compounds on the Growth of Aspergillus flavus and Related Aflatoxin B1 Production: A Review
International Journal of …, 2022
[2] Fungal Secondary Metabolites-Fungal Markers for the Indoor Air
2022
[3] Volatile Organic Compounds Emitted by Aspergillus flavus Strains Producing or Not Aflatoxin B1
Toxins, 2021
[4] 褐色腐朽菌が木材腐朽過程で放散する揮発性有機化合物の多様性
2020
[5] Methods for Early Detection of Microbiological Infestation of Buildings Based on Gas Sensor Technologies
2020
[6] Fingerprinting ambient air to understand bioaerosol profiles in three different environments in the south east of England
2020
[7] Assessment of volatile metabolites for in situ detection of fungal decay in wooden structures
2020
[8] Application of Gas Chromatography–Mass Spectroscopy (GC-MS) and Electronic Noses for Detection of Aflatoxin Contamination of Maize
2019
[9] Pathogenic Variability among Macrophomina Phaseolina Isolates Associated With Soybeans
International Journal of Life Sciences Research, 2018
[10] Volatile Metabolomics with Focus on Fungal and Plant Applications-A Review
2018
[11] Characterizing outdoor air using microbial volatile organic compounds (MVOCs)
2018
[12] Sniffing out the hypoxia volatile metabolic signature of Aspergillus fumigatus
Journal of Breath Research, 2017
[13] Mass spectrometric techniques for the analysis of volatile organic compounds emitted from bacteria
2017
[14] Assessment of Volatile Metabolites for In Situ Detection of Fungal Decay of Wood
ProQuest Dissertations Publishing, 2017
[15] Effects of Growth Parameters on the Analysis of Aspergillus flavus Volatile Metabolites
Separations, 2016
[16] 真菌類に由来する揮発性有機化合物の木材保存への可能性
木材保存, 2016
[17] Fingerprinting outdoor air environment using microbial volatile organic compounds (MVOCs)-A review
TrAC Trends in Analytical Chemistry, 2016
[18] Ian Colbeck 2, Diogo Gomes, Sean Tyrrel and Frederic Coulon 5 6 1Cranfield University, School of Water, Energy and Environment, Cranfield, MK43 0AL, UK 7 …
2016
[19] 褐色腐朽菌オオウズラタケが放散する揮発性有機化合物の分析手法の検討
木材保存, 2015
[20] Multivariate Analysis of Microbial Volatile Organic Compounds for Aflatoxigenic A. flavus Detection
2015
[21] Analysis of complex volatile organic compound mixtures using active SPME-GC-MS
2015
[22] Automated Template C: Created by James Nail 2013V2.
2015
[23] Multivariate analysis of fungal volatile metabolites for aflatoxigenic fungi detection
ProQuest Dissertations Publishing, 2015
[24] 固相微萃取 (SPME) 近几年的发展
分析试验室, 2015
[25] Automated Template B: Created by James Nail 2011V2.
2015

Copyright © 2024 by authors and Scientific Research Publishing Inc.

Creative Commons License

This work and the related PDF file are licensed under a Creative Commons Attribution 4.0 International License.