American Journal of Molecular Biology
Volume 4, Issue 1 (January 2014)
ISSN Print: 2161-6620 ISSN Online: 2161-6663
Google-based Impact Factor: 1.47 Citations
A method to synthesize cDNA constructs by homology based recombination cloning ()
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ABSTRACT
We introduce a homology-based recombination approach for generating a cDNA construct. This method depends on amplifying several exon fragments and their fusions by the homology-based recombination. This method provides a way to generate the cDNA sequence of any gene without any need for its mRNA. The paper describes the strategy by assembling cDNA of the MYB1 and MYB2 genes of Arabidopsis thaliana.
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