Journal of Analytical Sciences, Methods and Instrumentation

Volume 3, Issue 4 (December 2013)

ISSN Print: 2164-2745   ISSN Online: 2164-2753

Google-based Impact Factor: 0.42  Citations  

Optical Rotatory Dispersion Measurement of D-Glucose with Fixed Polarizer Analyzer Accessory in Conventional Spectrophotometer

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DOI: 10.4236/jasmi.2013.34030    7,168 Downloads   12,815 Views  Citations
Author(s)

ABSTRACT

In the sample compartment of a conventional spectrophotometer, mounting of a polarizer before sample and an analyzer behind sample allows the determination of the optical rotatory dispersion of optical active media by measurement of the transmission ratio of crossed and parallel arranged polarizer and analyzer. A formula for the determination of the angle of rotation is derived from the transmission ratio. The arrangement is applied to determine the molar optical rotation of D-glucose in water in the wavelength range from 220 nm to 820 nm.

Share and Cite:

A. Penzkofer, "Optical Rotatory Dispersion Measurement of D-Glucose with Fixed Polarizer Analyzer Accessory in Conventional Spectrophotometer," Journal of Analytical Sciences, Methods and Instrumentation, Vol. 3 No. 4, 2013, pp. 234-239. doi: 10.4236/jasmi.2013.34030.

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