Engineering

Volume 5, Issue 10 (October 2013)

ISSN Print: 1947-3931   ISSN Online: 1947-394X

Google-based Impact Factor: 0.79  Citations  

Cloning, Characterization and Bioinformatic Analysis of the Gene Encoding the Larval Serum Protein 2 in Diapause of the Onion Maggot, Delia Antiqua

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DOI: 10.4236/eng.2013.510B100    3,888 Downloads   4,771 Views  

ABSTRACT

The full-length cDNA encoding Larval serum protein 2 (LSp-2) in the onion maggot,Delia antiqua, was cloned and sequenced by rapid ampli?cation of cDNA ends methods. The result showed that the cDNA was 2203 bp long and the open reading frame (ORF) of 2106 bp encoded 701 amino acid with a calculated molecular weight of 80.5 kDa and an isoelectric point of 5.87. The onion maggot LSp-2 shows highest homology (83%) to that ofCalliphora vicinaat amino acid level. Its signal peptides, domains and structures were predicted and analyzed by using bioinformatic methods. The amino acid sequence of LSP-2 suggests that it would be a typical hexamerin.

Cite this paper

Xu, J. , Chen, B. , He, Z. and Hao, Y. (2013) Cloning, Characterization and Bioinformatic Analysis of the Gene Encoding the Larval Serum Protein 2 in Diapause of the Onion Maggot, Delia Antiqua. Engineering, 5, 487-490. doi: 10.4236/eng.2013.510B100.

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