The study aimed to evaluate the instrumental and clinical properties of an innovative gel formulation for anti-aging treatment. This was an open, non-controlled study, where the eligible subjects, divided into three subgroups according to age, had to perform a single dose application of the gel on the face for a short-term evaluation and a 4-week repeated use, twice a day, for a long-term evaluation. Instrumental and clinical evaluations had to be performed mono-laterally at the level of the face (right or left side according to a predisposed randomization list) in basal conditions (T₀), 20 minutes after the first dose application (T_20min) and after 2 and 4 weeks of treatment (T_2wks - T_4wks). Thirty-three subjects completed the study showing, after only 20 minutes from the 1^st product application, a clinically important and statistically significant improvement of crow’s feet, skin dullness clinical score and cutaneous microrelief clinical score (p < 0.05), after 2 weeks of treatment an improvement of skin tonicity (p < 0.05) and after 4 weeks of treatment having a significant decrease of nasolabial folds clinical score (p < 0.05). The study showed the great efficacy of an innovative anti-aging gel in reducing the skin roughness and skin dryness, improving the skin firmness and, in general, providing a global skin rejuvenation.