The lack of efficient application of transportation planning process in developing cities, such as Gaza, leads to deficiency in adopting the suitable transport policies to mitigate the transportation problems resulting from urbanization and rapid increase of population. The mode choice model is probably the most important element in transportation planning and policy making. The aim of this study is to develop mode choice model for work trips in Gaza city and therefore investigating the factors that affect the employed people’s choice for transport modes. The model was developed using about two thirds of 552 questionnaires distributed for this purpose. The rest remaining third of questionnaires were used to validate the chosen models. The results of this research show that the factors that significantly affect the choice of transport modes are: total travel time, total cost divided by personal income, ownership of means of transport, distance, age, and average family monthly income. The developed model is able to predict the choice behavior of employed people in Gaza city as it is valid at 95% confidence level. This study can be used by transportation planners to predict the employed people’s behavior and travel demand analysis. The developed model can be used for predicting the future modal split by inputting predicted future value of exploratory variables.