Author(s)

Mai Kobayashi, Isao Matsui-Yuasa, Maiko Fukuda-Shimizu, Yoshinobu Mandai, Masaki Tabuchi, Hiroshi Munakata, Akiko Kojima-Yuasa

Department of Biochemistry, Kinki University Medical School, Osaka-Sayama, Japan.
Department of Food and Human Health Sciences, Graduate School of Human Life Science, Osaka City University, Osaka, Japan.
Nitta Gelatin Inc., Yao, Japan.

Mangoes (Mangifera indica L.) are one of the most important tropical foods. The seed is one of the main by-products of mango processing. Therefore, it is important to find an economically viable use for this waste (e.g., as a food additive or supplement with high nutraceutical value). We investigated the anti-obesity effects of mango seed kernel extract with hot water (MSKE-W) in 3T3-L1 adipocytes and in a high fat diet (HFD)-induced obesity rat model. MSKE-W caused a significant decrease in the activity of glycerol 2-phosphate dehydrogenase in 3T3-L1 adipocytes without eliciting cell cytotoxicity and inhibited cellular lipid accumulation through down-regulation of transcription factors such as PPARγ and C/EBPα. In the animal model, rats fed an HFD containing 1% MSKE-W gained less weight than rats fed an HFD alone. The visceral fat mass in rats fed an HFD containing 1% MSKE-W tended to be lower than that in rats fed an HFD alone. Furthermore, histological examination of rat livers from an HFD showed steatohepatitis. However, rats on an HFD containning 1% MSKE-W showed no histopathological changes in liver tissue. Our results indicate that MSKE-W influences anti-obesity effects, both in vitro and in vivo, and suggest that MSKE-W provides a novel preventive potential against obesity.

Mago Seed Kernel; Glycerol 2-Phosphate Dehydrogenase (GPDH); Peroxisome Proliferator-Activated Receptor γ (PPARγ); High Fat Diet-Induced Obesity Rat Model; 3T3-L1 Adipocytes

Kobayashi, M. , Matsui-Yuasa, I. , Fukuda-Shimizu, M. , Mandai, Y. , Tabuchi, M. , Munakata, H. and Kojima-Yuasa, A. (2013) Effect of mango seed kernel extract on the adipogenesis in 3T3-L1 adipocytes and in rats fed a high fat diet. Health, 5, 9-15. doi: 10.4236/health.2013.58A3002.