A regional map of mangrove forests was produced for six islands located in the southern part of Japan by integrating the spectral analyses of Landsat Enhanced Thematic Mapper plus (ETM+) images with a digital elevation model (DEM). Several attempts were applied to propose a reliable method, which can be used to map the distribution of mangrove forests at a regional scale. The methodology used in this study comprised of obtaining the difference between Normalized Difference Water Index (NDWI) and Normalized Difference Vegetation Index (NDVI), band ratio 5/4, and band 5, from Landsat ETM+, and integrating them with the topographic information. The integration of spectral analyses with topographic data has clearly separated the mangrove forests from other vegetation. An accuracy assessment was carried out in order to check the accuracy of the results. High overall accuracy ranging from 89.3% to 93.6% was achieved, which increased the opportunity to use this methodology in other countries rich in mangrove forests.