A total of 14 parts of beef cuts were used to determine the amino acid composition in drought-master beef. Droughtmaster beef is a cross breed tropical cattle with 50% Shorthorn and 50% Brahman cattle. The most abundant type of amino acid in droughtmaster beef was glutamic acid, followed by aspartic acid, lysine, leucine and arginine. The flank cut of beef was found to contain higher amount of total amino acids followed by top side and short rib cut of beef. The amino acid composition of droughtmaster beef indicates that beef is a good source of dietary protein for human.