Advances in Bioscience and Biotechnology

Volume 4, Issue 4 (April 2013)

ISSN Print: 2156-8456   ISSN Online: 2156-8502

Google-based Impact Factor: 1  Citations  h5-index & Ranking

Detection of cardiac myosin binding protein-C (cMyBP-C) by a phospho-specific PKD antibody in contracting rat cardiomyocytes

HTML  XML Download Download as PDF (Size: 1650KB)  PP. 1-6  
DOI: 10.4236/abb.2013.44A001    3,766 Downloads   6,751 Views   Citations


Protein phosphorylation plays an important role in physiological processes, such as muscle contraction. Phospho-specific antibodies have become powerful tools to study these processes. Cardiac myosin binding protein-C (cMyBP-C) is one of the proteins that make up the contractile apparatus of cardiomyocytes. Phosphorylation of cMyBP-C is essential for normal cardiac function, since dephosphorylation of this protein leads to its degradation and has been associated with cardiomyopathy. One of the upstream kinases, which phosphorylate cMyBP-C, is protein kinase D (PKD). While studying the role of PKD in cMyBP-C phosphorylation, we tried to analyze phosphorylation of PKD with a phospho-specific PKD-Ser744/748 antibody. Contrary to the expected 115 kDa, a signal was found for a 150-kDa protein. By MALDI-TOF mass spectrometry, we identified this protein to be cMyBP-C. These data were confirmed by immunostaining using the p-PKD-Ser744/748 antibody, which displayed a striated pattern similar to the one observed for a regular cMyBP-C antibody. To our knowledge there are no antibodies commercially available for phosphorylated cMyBP-C. Thus, the p-PKD-Ser744/748 antibody can accelerate research into the role of cMyBP-C phosphorylation in cardiomyocytes.

Cite this paper

Dirkx, E. , Bouwman, F. , Vertommen, D. , Mariman, E. , Sadayappan, S. , Glatz, J. , Luiken, J. and Eys, G. (2013) Detection of cardiac myosin binding protein-C (cMyBP-C) by a phospho-specific PKD antibody in contracting rat cardiomyocytes. Advances in Bioscience and Biotechnology, 4, 1-6. doi: 10.4236/abb.2013.44A001.

Cited by

[1] Cardiac contraction-induced GLUT4 translocation requires dual signaling input
Trends in Endocrinology & Metabolism, 2015
[2] 杨树蛋白质磷酸化位点预测
生物信息学, 2015

Copyright © 2020 by authors and Scientific Research Publishing Inc.

Creative Commons License

This work and the related PDF file are licensed under a Creative Commons Attribution 4.0 International License.