The study investigated the psychological characteristics of entrepreneurship of 829 college students in Chinaby questionnaire. The psychological characteristics include five aspects: entrepreneurial capacity, entrepreneurial belief, entrepreneurial consciousness, entrepreneurial motivation, and entrepreneurial determination. In the five factors, the entrepreneurial consciousness of college students is the highest, and the other four are almost at the same level. There is significant difference on entrepreneurial belief, consciousness, and motivation in different gender of students. Also there is significant difference on entrepreneurial consciousness in different major students and on entrepreneurial motivation in different degree students. However, the college students only with high entrepreneurial consciousness are not enough to succeed in business. The other four psychological characteristics are not only essential to entrepreneurship, but also necessary to their future professional development. College students should have a lot of preparation and accumulation on the other four psychological characteristics of entrepreneurship, and give much more effort to improve them.