Author(s)

Hironori Ushijima, Masatomo Maeda

Department of Molecular Biology, School of Pharmacy, Iwate Medical University, Shiwa-Gun, Japan.

We screened 95 kinase inhibitors whether they affect cAMP-dependent proteolysis of GATA-6 or not. Among them 7 inhibitors inhibited the proteolysis at the concentration range of μM around their IC50. They are inhibitors for protein kinase A (H-89 and 4- cyano-3-methylisoquinoline), c-Jun N-terminal kinase (SP600125), phosphatidylinositol 3-kinase (Wort- mannin and LY-294002), casein kinase II (TBB) and cyclin dependent kinase (Cdk1/2 inhibitor III). It is of interest how these kinases play roles in the degradation process of GATA-6 since this transcription factor is essential for development and tissue-specific gene expression of mammals. Inhibitors identified in this study would be helpful to study molecular mechanisms of phenomena in which GATA-6 participates.

cAMP; GATA-6; Kinase Inhibitor; Proteolysis; Transcription Factor

Ushijima, H. and Maeda, M. (2012) Inhibitors of protein kinases affecting cAMP-dependent proteolysis of GATA-6. Advances in Biological Chemistry, 2, 411-415. doi: 10.4236/abc.2012.24051.