Health

Volume 4, Issue 10 (October 2012)

ISSN Print: 1949-4998   ISSN Online: 1949-5005

Google-based Impact Factor: 0.74  Citations  

Development of H5 subtype-specific monoclonal antibodies (MAb) and MAb-based assays for rapid detection of H5 avian influenza

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DOI: 10.4236/health.2012.430141    4,409 Downloads   7,094 Views  Citations

ABSTRACT

Avian influenza (AI) virology surveillance is the most important method to monitor AI virus (AIV) in poultry so as to effectively prevent and control AI outbreaks. Monoclonal antibodies (MAb)-based assays are highly sensitive and specific for AIV detection, and much practical and economic for test-in-field or onsite. Many such assays have been developed and are still in developing since the H5N1 highly pathogenic AI (HPAI) outbreaks occurred in South East Asia in 2003. A MAb-based dot-enzyme-linked immunosorbent assay (ELISA) has been developed in our lab during late 1990s and early 2000s. Meanwhile, AIV H7 and H5 subtype specific-MAbs have been successfully developed in our laboratory to enhance the Dot-ELISA and other MAb-based assays for AIV detection. Production and purification of the H7 and H5 MAbs were made to provide essential reagents for Dot-ELISA and other immunoassays, and the current development of a novel Biosensor technique for rapid detection of AIV from clinical and field specimens.

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Lu, H. , Lin, L. , Wang, R. , Li, Y. , Li, Y. , Scheuchenzuber, B. , Liu, J. , Xie, Z. and Rosebrock, J. (2012) Development of H5 subtype-specific monoclonal antibodies (MAb) and MAb-based assays for rapid detection of H5 avian influenza. Health, 4, 923-926. doi: 10.4236/health.2012.430141.

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