Author(s)

Kowichi Jimbow, Yasuaki Tamura, Akihiro Yoneta, Takafumi Kamiya, Ichiro Ono, Toshiharu Yamashita, Akira Ito, Hiroyuki Honda, Kazumasa Wakamatsu, Shosuke Ito, Satoshi Nohara, Eiichi Nakayama, Takeshi Kobayashi

Department of Biological Chemistry, College of Bioscience and Biotechnology, Chubu University, Ka- sugai, Japan..
Department of Biotechnology, School of Engineering, Nagoya University, Nagoya, Japan.
Department of Chemical Engineering, Faculty of Engineering, Kyushu University, Fukuoka, Japan.
Department of Chemistry, Fujita Health University School of Health Sciences, Toyoake, Japan.
Department of Dermatology, Sapporo Medical University School of Medicine, Sapporo, Japan.
Department of Dermatology, Sapporo Medical University School of Medicine, Sapporo, Japan;.
Faculty of Health and Welfare, Kawasaki University of Medical Welfare, Kurashiki, Japan.
Institute of Dermatology & Cutaneous Sciences, Sapporo, Japan.
Meito Sangyo Co., Nagoya, Japan.
Sapporo Medical University School of Medicine, Sapporo, Japan.

In order to develop melanoma-targeted in situ peptide vaccine immunotherapy, magnetite nanoparticles were conjugated with a melanogenesis substrate, N-propionyl cysteaminylphenol (NPrCAP). Magnetite nanoparticles introduced thermotherapy which caused non-apoptotic cell death and generation of heat shock protein (HSP) upon exposure to alternating magnetic field (AMF). NPrCAP was expected to develop a melanoma-targeted therapeutic drug because of its selective incorporation into melanoma cells and production of highly reactive free radicals, that result in not only oxidative stress but also apoptotic cell death by reacting with tyrosinase.

Melanogenesis; Chemo-Thermo-Immunotherapy; Nanomedicine; Melanoma; Magnetite Nanoparticles

K. Jimbow, Y. Tamura, A. Yoneta, T. Kamiya, I. Ono, T. Yamashita, A. Ito, H. Honda, K. Wakamatsu, S. Ito, S. Nohara, E. Nakayama and T. Kobayashi, "Conjugation of Magnetite Nanoparticles with Melanogenesis Substrate, NPrCAP Provides Melanoma Targeted, in Situ Peptide Vaccine Immunotherapy through HSP Production by Chemo-Thermotherapy," Journal of Biomaterials and Nanobiotechnology, Vol. 3 No. 2, 2012, pp. 140-153. doi: 10.4236/jbnb.2012.32020.