Open Access Library Journal

Volume 8, Issue 3 (March 2021)

ISSN Print: 2333-9705   ISSN Online: 2333-9721

Google-based Impact Factor: 0.47  Citations  

Production of α-Amylase by Bacillus subtilis QM3 and its Enzymatic Properties

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DOI: 10.4236/oalib.1107291    71 Downloads   238 Views  

ABSTRACT

Alpha-amylase is a starch hydrolyzing enzyme which has many industrial applications. In this study, Bacillus subtilis QM3 has ability to produce α-amylase. Identification was done by iodine test, based on the clear zone around the sample in starch agar plates. Alpha-amylase activity was measured using 3,5-dinitrosalicylic acid (DNS). The result showed B. subtilis QM3 can produce α-amylase and activity of α-amylase was 50.58 IU/mL. The study on enzymatic properties was found: the optimum temperature is 70°C, and its stability is high at 30°C - 70°C. The optimum pH is 6.0, and the stability is high under the condition of pH was 6.0 - 7.0. Ca2+, Na+, K+ and Mg2+ promoted the activity of alpha-amylase, and their relative enzyme activities were 130.1%, 118.1%, 115.9% and 110.4% respectively. Fe2+, Zn2+, Cu2+, Mn2+ and EDTA inhibited the activity of alpha-amylase. The relative enzyme activities were 86.1% 77.8%, 67.4%, 64.2% and 48.4% respectively.

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Hu, Q.P. and Liu, J.J. (2021) Production of α-Amylase by Bacillus subtilis QM3 and its Enzymatic Properties. Open Access Library Journal, 8, 1-8. doi: 10.4236/oalib.1107291.

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