Author(s)

Safaâ Rahmouni^1*, Zineb Nejjar El Ansari¹, Alain Badoc², Patrick Martin³, Mohammed L’Bachir El Kbiach¹, Ahmed Lamarti¹

¹Laboratory of Plant Biotechnology, Department of Biology, Faculty of Sciences, Abdelmalek Essaadi University, Tetouan, Morocco.
²Axe MIB (Molécules d’Intérêt Biologique), Unité de Recherche Œnologie, UFR des Sciences Pharmaceutiques, Université de Bordeaux, ISVV (Institut des Sciences de la Vigne et du Vin), Villenave-d’Ornon, France.
³Université d’Artois, UniLaSalle, Unité Transformations & Agroressources, Béthune, France.

In the present study, we tested the effect of amino acids on secondary somatic embryogenesis of Moroccan cork oak (Quercus suber L.). Secondary mature and immature somatic embryos were obtained from primary somatic embryos cultured in N₃₀K medium supplemented with nineteen amino acids. Stimulation of embryogenesis was dependent on the type and concentration of amino acid in the medium. Thus, L-glutamine and L-asparagine at 3.42 mM have been proved to be the most favorable for the formation of functional somatic embryos and the induction of secondary somatic embryos.

Cork Oak, Quercus suber L., Secondary Somatic Embryogenesis, Amino Acids

Rahmouni, S. , El Ansari, Z. , Badoc, A. , Martin, P. , El Kbiach, M. and Lamarti, A. (2020) Effect of Amino Acids on Secondary Somatic Embryogenesis of Moroccan Cork Oak (Quercus suber L.) Tree. American Journal of Plant Sciences, 11, 626-641. doi: 10.4236/ajps.2020.115047.