Author(s)

Md. Nur Hossain^1*, Annana Akter², Sanjida Humayan¹, Liton Chandra Mohanto¹, Shamima Begum², Monzur Morshed Ahmed¹

¹Institute of Food Science and Technology, Bangladesh Council of Scientific and Industrial Research, Dhaka, Bangladesh.
¹Institute of Food Science and Technology, Bangladesh Council of Scientific and Industrial Research，Dhaka, Bangladesh.
²Department of Microbiology, Jagannath University, Dhaka, Bangladesh.

Conventional growth media for microbes contains lots of non-edible components that are harmful to consume when the organism is ready to intake—like probiotics growing in chemical media (Lactobacillus grown in MRS media). The study was conducted to develop an edible and low-cost growth media that supports the growth of probiotics Lactic Acid bacteria for the enhancement of probiotic research. The 04 isolates of Lactobacillus (L. plantarum, L. rhamnosus, L. ferciminis and L. bifarmentans) and 02 Bifidobacterium (B. infantis and B. Bifidum) were used for the evaluation of medium efficacy. To formulate the edible culture media, 04 vegetables and 02 pulses were used. The media was formulated in different formulations. For Lactobacillus, maximum growth was observed at MM-2 media formulation that was about 15.62 log CFU/ml for L. plantarum and compared to MRS media that was 11.78 log CFU/ml. Bifidobaterium showed the highest viability at MW-1 edible media formulation which was about 12.72 log CFU/ml whereas in Bifidobacteria selective media the cell viability was 12.48 log CFU/ml. The edible media has no toxic or unhealthy effect on mice while trailing in an animal model and shows excellent results in encapsulation with alginate. In comparison with the performance of traditional chemical media, the formulated media were found to be cost-effective and safe for human consumption.