Author(s)

Agnes Antoinette Ntoumba¹, Francois Eya’ane Meva^2,3*, Wolfgang Eyisap Ekoko^1,4, Loick Pradel Kojom Foko¹, Etoile Ngo Hondt¹, Carsten Schlüsener³, Bastian Moll³, Gisele Etame Loe², Philippe Belle Ebanda Kedi¹, Jean Yves Sikapi Fouda², Christoph Janiak³, Leopold Gustave Lehman¹

¹Department of Animal Biology and Physiology, Faculty of Science, The University of Douala, Douala, Cameroon.
²Department of Pharmaceutical Sciences, Faculty of Medicine and Pharmaceutical Sciences, The University of Douala, Douala, Cameroon.
³Institute for Inorganic Chemistry and Structural Chemistry, Heinrich-Heine-University Düsseldorf, Universitätsstr 1, Düsseldorf, Germany.
⁴Organisation de Coordination pour la lutte contre les Endémies en Afrique Centrale (OCEAC), Yaoundé, Cameroon.

The progress in the field of nanotechnology has contributed to the development of tools for combating the most critical problems in developing countries. The requirements that such tools should meet are low-cost and resource settings, environmental protection, ease of use, and availability. The use of plant properties for the generation of nanoparticles (NPs), which serve as bioinsecticides to combat the plasticity and resistance of mosquitoes and parasites, is considered possible. Here, we report for the first time the larvicidal activity of silver (Ag) NPs (AgNPs) synthesized from Psidium guajava (P. guajava) extract, which targets the 4^th instar larvae of Anopheles gambiae. Concentrations of AgNPs between 0 and 200 ppm were used and their LC₅₀ at 24 h and 48 h were determined as 19.55 ppm and 8.737 ppm, respectively. The AgNPs were stable and highly effective against the larvae of A. gambiae and thereby we anticipate that they can be used to combat vector-borne diseases in developing countries.

Vector Control, Anopheles gambiae, Silver Nanoparticles, Psidium guajava

Ntoumba, A. , Meva, F. , Ekoko, W. , Foko, L. , Hondt, E. , Schlüsener, C. , Moll, B. , Loe, G. , Kedi, P. , Fouda, J. , Janiak, C. and Lehman, L. (2020) Biogenic Synthesis of Silver Nanoparticles Using Guava (Psidium guajava) Leaf Extract and Its Larvicidal Action against Anopheles gambiae. Journal of Biomaterials and Nanobiotechnology, 11, 49-66. doi: 10.4236/jbnb.2020.111004.