Mutational and Phylogenetic Analysis of nfxB Gene in Multidrug-Resistant Clinical Isolates of Pseudomonas aeruginosa Hyperexpressing MexCD-OprJ Efflux Pump

HTML  XML Download Download as PDF (Size: 580KB)  PP. 993-999  
DOI: 10.4236/aim.2019.912064    551 Downloads   1,379 Views  Citations

ABSTRACT

The present study focused on MexCD-OprJ efflux pump and its regulatory gene nfxB in multidrug resistant (MDR) clinical isolates of Pseudomonas aeruginosa collected from Kerala, South India. Semi-quantitative reverse transcription-PCR technique was employed to detect hyperexpression of the efflux pump gene, mexD. Amplicons from nfxB gene of isolates hyperexpressing the efflux pump were sequenced for mutational and phylogenetic analysis. Among 29 isolates of MDR P. aeruginosa, increased mexD transcription was detected in 10.3% of the isolates when compared with P. aeruginosa reference strain, PAO (MTCC-3541). Various synonymous and non-synonymous mutations in nfxB regulatory gene sequences were detected. Notably, mutations detected in the strains designate Pa6 and Pa7 have been found to be novel and are hitherto unreported in GenBank data base. The genetic divergence and homogeneity of the nfxB regulatory gene sequences of mexCD-oprJ operon were clearly apparent in the phylogram generated employing similar sequences retrieved from the public database.

Share and Cite:

Suresh, M. , Narayanan, N. , Puthurath Vimal, K. , Jayasree, P. and Manish Kumar, P. (2019) Mutational and Phylogenetic Analysis of nfxB Gene in Multidrug-Resistant Clinical Isolates of Pseudomonas aeruginosa Hyperexpressing MexCD-OprJ Efflux Pump. Advances in Microbiology, 9, 993-999. doi: 10.4236/aim.2019.912064.

Copyright © 2024 by authors and Scientific Research Publishing Inc.

Creative Commons License

This work and the related PDF file are licensed under a Creative Commons Attribution 4.0 International License.