Author(s)

Xing Chen^1,2*, Shujun Zhang^1,2, Zhangrui Cheng², Geoffrey E. Pollott², D. Claire Wathes²

¹Key Laboratory of Agricultural Animal Genetics, Breeding, and Reproduction of Ministry of Education, Huazhong Agricultural University, Wuhan, China.
²Department of Pathobiology and Population Sciences, Royal Veterinary College, Hatfield, UK.

Mastitis reduces milk production and causes culling. The NF-κB transcription factor RelA plays a central regulatory role in innate immunity. This study used a candidate gene approach to investigate associations between the synonymous C/G SNP rs48035703 in RELA with somatic cell count (SCC) and survival time. Blood samples were collected from 337 Holstein-Friesian heifers on 19 farms and genotyped by PCR-restriction fragment length polymorphism. Animals were monitored from 6 months until 2340 d of age. Pedigree, milk production and disease records were obtained. Genotype frequencies were CC 0.63, CG 0.30 and GG 0.06. The C allele had a favourable additive effect on survival: average longevities from birth were CC, 1872 d; CG, 1745 d and GG 1596 d (P < 0.003). Log transformed first lactation somatic cell count (SCC)data showed a significant association with this SNP using an allele substitution model (mean residuals ± SD: GG 0.30 ± 1.263; CG 0.22 ± 0.994, CC -0.04 ± 0.803, P < 0.05). More CC cows than expected were classified as intermediate and fewer as mastitic (30.4% v 45.9%) with respect to SCC class when categorised as 0 (unaffected), 1 (intermediate) and 2 (mastitic), whereas for CG heterozygotes fewer were intermediate and more were mastitic (12.1% v 60.3%) (p = 0.05). RELA rs48035703 CC genotype cows were therefore less likely to experience a high SCC and survived longer. These results support a role for RelA in combating mammary gland infection and warrant further studies in additional populations.

Mastitis, Longevity, RelA, NF-κB, Dairy Cow

Chen, X. , Zhang, S. , Cheng, Z. , Pollott, G. and Wathes, D. (2019) Association between Single Nucleotide Polymorphism in RelA with Somatic Cell Count and Longevity Supports Importance of NF-κB Signalling in Cattle Health. Open Journal of Animal Sciences, 9, 51-64. doi: 10.4236/ojas.2019.91005.