Author(s)

Mohammad Naser Haidary^*, Tomoko Tamura, Makoto Ueno^*

Laboratory of Plant Pathology, Faculty of Life and Environmental Science, Shimane University, Matsue, Japan.

Microorganism isolates (n = 49) were obtained from the soil samples collected from field in Gotsu city (Kawahira), Shimane. Isolate GT2-E culture inhibited the growth of Xanthomonas oryzae pv. oryzae in disk diffusion method. Rice bacterial leaf blight was suppressed by GT2-E culture in the pre- and post-treated rice leaves. Sequence analysis of 16S rDNA region of the GT2-E isolate indicated that it shared 99% similarity with Paenibacillus polymyxa. The growth of GT2-E on LB medium was observed at 15°C, 28°C, 37°C, and 45°C, but not at 4°C. GT2-E isolate could be grown even in the presence of agrochemicals (Amister, Blasin and Kasumin). Furthermore, the growth of X. oryzae pv. oryzae was inhibited by the culture filtrate of GT2-E isolate in disk diffusion method. However, the inhibitory activity of the culture filtrate was heat-unstable. This result suggested that GT2-E isolate can produce heat-unstable inhibitory compound(s). In conclusion, GT2-E isolate might contribute to the development of a new bactericide and biological agent against rice bacterial leaf blight.

Rice Bacterial Leaf Blight, Xanthomonas oryzae pv. oryzae , Microorganisms, Paenibacillus polymyxa

Haidary, M. , Tamura, T. and Ueno, M. (2018) Inhibitory Activity of Paenibacillus sp. Isolated from Soil in Gotsu City, Shimane Prefecture, Against Xanthomas oryzae pv. oryzae, the Causal Agent of Rice Bacterial Leaf Blight. Advances in Microbiology, 8, 197-210. doi: 10.4236/aim.2018.83014.