Genetic Variations and Relationships of Papua’s Endemic Orchids Based on RAPD Markers - Natural Science

NS > Vol.9 No.11, November 2017

Genetic Variations and Relationships of Papua’s Endemic Orchids Based on RAPD Markers ()

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DOI: 10.4236/ns.2017.911035 1,400 Downloads 3,033 Views Citations

Author(s)

Barahima Abbas¹, Muhammad Dailami², Florentina Heningtyas Listyorini¹, Munarti ³

Affiliation(s)

¹Faculty of Agriculture, University of Papua (UNIPA), Manokwari, Indonesia.
²Faculty of Mathematics and Science, University of Papua (UNIPA), Manokwari, Indonesia.
³Faculty of Teacher Training and Education Science, The Pakuan University, Bogor, Indonesia.

ABSTRACT

Orchidaceae has known as an attractive flower and immense species. We have found a large species of Orchidaceae grow naturally in Papua’s jungle, Indonesia territorial. This study aims to reveal genetic variation and genetic relationships among endemic orchids in Papua based on RAPD markers. The study included 26 accessions of Papua’s endemic orchids used for genomic DNA extraction. Genomic DNAs were extracted by usingDNAextraction kit from Qiagen and genomic DNA amplification by using 10 decamer RAPD primers. DNA fragments that were amplified by Polymerase Change Reaction (PCR) were visualized and documented by using UV illumination apparatus. Genetically, endemic Orchids in Papua were described high variation. Fragments amplification by using ten RAPD primers and performed in the PCR tools resulted in 54 numbers of polymorphic fragments and no monomorphic band. The number of polymorphic bands per primer ranged from 4 to 7 with averaged 5.4 bands per assay unit. The genetic dissimilarities (GDs) among examined orchids ranged from 0.10 to 0.94 based on Nei’s unbiased coefficients. Dendrogram construction showed that Papua’s endemic orchid (PEO) samples different from another and separated to form group by their own at the 0.40 coefficient value and at the 0.6 coefficient value indicate that PEO sample is divided into nine groups i.e. samples at the genera level were separated into their own groups.

KEYWORDS

Orchidaceae, Genetic Variation, Genetic Relationships, Papua’s Endemic, RAPD

Share and Cite:

Abbas, B. , Dailami, M. , Listyorini, F. and , M. (2017) Genetic Variations and Relationships of Papua’s Endemic Orchids Based on RAPD Markers. Natural Science, 9, 377-385. doi: 10.4236/ns.2017.911035.

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