Author(s)

Chiara Castellini, Silvana Belletti, Paolo Govoni, Stefano Guizzardi

Department of Biomedical, Biotechnological and Translational Sciences—Section of Histology, University of Parma, Parma, Italy.

Skin aging and most age-related diseases are associated with a low-grade chronic inflammation. The nucleoside adenosine, a potent endogenous anti-inflammatory agent, is deeply involved in inflammatory diseases and, by interaction with the adenosine A₂ receptor (A_2AR) it immediately promotes a mechanism of defence against the inflammatory damage. The aim of our study was to investigate whether polydeoxyribonucleotide (PDRN), a mixture of deoxyribonucleotides polymers of different lengths that like adenosine, binds the A_2A receptor, can reduce the inflammatory state in the macrophage cell line. RAW264.7, murine macrophage cells, were incubated with PDRN in the presence and in the absence of lipopolysaccaride (LPS), which was the major component of the outer membrane of gram-negative bacteria and which acted as a strong macrophage activator. We assessed the production of nitric oxide and the secretion of inflammatory mediators (i.e., TNF-α, IL-10, IL-12 and VEGF-A). Our data showed that PDRN produced a significant decrease of inflammation in macrophages pre-stimulated with LPS, assessed in terms of the nitric oxide content (p < 0.001) and cytokines secretion (p < 0.001). Moreover, PDRN stimulated the release of the vascular endothelial growth factor (VEGF-A), which promoted wound healing. Our study suggested that PDRN, by binding the A_2A receptor, contributed to a great extent towards reducing inflammation.

Cell Culture, Macrophages, RAW264.7, Inflammation, Skin Aging

Castellini, C. , Belletti, S. , Govoni, P. and Guizzardi, S. (2017) Anti Inflammatory Property of PDRN—An in Vitro Study on Cultured Macrophages. Advances in Bioscience and Biotechnology, 8, 13-26. doi: 10.4236/abb.2017.81002.