According to the extent of fungal infections, to be chronic these such diseases and recently the emerging issue of increased antibiotic resistance in fungal infections, most of scientists are going to find a proper way to replace antibacterial agent by significant semiconductor ZnO nanoparticles (NPs). They are well known to be one of the most important and special metal oxide nanoparticles in pharmaceutical against the most common fungi. ZnO nanoparticles were synthesized using sol-gel, hydrothermal and functionalized surface methods and formulated in water solutions as nanofluids. XRD, FTIR and SEM techniques and UV-Vis absorbance spectroscopy characterized their ZnO modified nanostructures. Also antimycotic potential according to generally tests such as: (MIC) minimum inhibitory concentration, (MFC) minimum fungicidal concentration and normally well diffusion method with standard strains fungi were performed. Among five common fungi strains using in this research, new various ZnO nanofluids showed noticeable results for dermatophyte fungi like Trichophyton mentagrophytes, Microsporum gypseum, Microsporum canis, Candida albicans and Candid tropicalis which had un growth zones in order 70, 40, 35, 30 and 30 mm in comparing with Clotrimazole reference reagent: 30, 25, 25, 18 and 20 mm by well method. The performance of MIC for ZnO nanofluids on fungi was determined to be equal to 0.35, 3.12, 6.25, 6.25 and 6.25 μgr/ml and MFC of nanoproducts showed the 1.5, 12.5, 25, 25 and 25 μgr/ml. Therefore, the designed ZnO nanofluids could reveal the most effect on fungi which cause dermal (ringworm), mucosal (thrush) and vaginal infections, so we are able to apply these surface high energetic ZnO water-based nanofluid formulations as in vitro nanomedicine and nanohygiene for the first time.