A Protocol for Endophyte-Free Callus Tissue of the Grape Vitis aestivalis “Norton/Cynthiana” (Vitaceae) - Agricultural Sciences

AS > Vol.7 No.10, October 2016

A Protocol for Endophyte-Free Callus Tissue of the Grape Vitis aestivalis “Norton/Cynthiana” (Vitaceae) ()

HTML XML

Download as PDF (Size: 2858KB) PP. 680-692

DOI: 10.4236/as.2016.710064 1,875 Downloads 3,413 Views Citations

Author(s)

Aimee Wilson¹, Matthew Fuller¹, Shannon Smith¹, Tony V. Johnston², John D. Du Bois¹

Affiliation(s)

¹Department of Biology, Middle Tennessee State University, Murfreesboro, TN, USA.
²School of Agribusiness and Agriscience, Middle Tennessee State University, Murfreesboro, TN, USA.

ABSTRACT

Vitis aestivalis is used in commercial wine production. Vegetative propagation of V. aestivalis has shown a low success rate. Although plant tissue culture has been a successful method to propagate many species, V. aestivalis has not yet been reliably grown as pure callus culture due to a fungal endophyte that exists within the plant. This study reports a viable protocol for obtaining fungus-free tissue culture callus from V. aestivalis. Explant tissue was chosen from healthy, actively growing plants grown in a growth room and in a vineyard. Tissues were sterilized with a combination of isopropanol, bleach, and chlorine dioxide gas and plated onto media containing chlorothalonil. The results from this study suggest that in order to obtain endophyte-free callus tissue, vine explants are to be taken from plants grown in a growth chamber simulating springtime conditions, sterilized in a combination of alcohol, bleach, and chlorine dioxide, and plated on selection media containing an antifungal agent, such as chlorothalonil. This technique could potentially be used with plants that have associated endophytes or other contamination problems to establish callus tissue for research and/or commercial propagation efforts.

KEYWORDS

Chlorothalonil, Endophyte, Vitis aestivalis, Norton, Cynthiana

Share and Cite:

Wilson, A. , Fuller, M. , Smith, S. , Johnston, T. and Du Bois, J. (2016) A Protocol for Endophyte-Free Callus Tissue of the Grape Vitis aestivalis “Norton/Cynthiana” (Vitaceae). Agricultural Sciences, 7, 680-692. doi: 10.4236/as.2016.710064.

Journals Menu

Follow SCIRP

	+1 323-425-8868
	customer@scirp.org
	+86 18163351462(WhatsApp)
	1655362766

	Paper Publishing WeChat

Journals Menu

Home

About SCIRP

Service

Policies