Mannitol and Sorbitol Improve Uniformity of Adventitious Shoots Regeneration in Echinacea purpurea L. Moench - Journal of Biomedical Science and Engineering

JBiSE > Vol.9 No.10, September 2016

Mannitol and Sorbitol Improve Uniformity of Adventitious Shoots Regeneration in Echinacea purpurea L. Moench ()

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DOI: 10.4236/jbise.2016.910B008 2,075 Downloads 3,978 Views Citations

Author(s)

Xiaolu Chen^1,2*, Dongliang Li^1,3, Yuesheng Yang^1*, Hong Wu¹

Affiliation(s)

¹College of Life Sciences, South China Agricultural University, Guangzhou, China.
²Tropical Crops Genetic Resources Institute, Chinese Academy of Tropical Agricultural Sciences (CATAS), Danzhou, China.
³Institute of Tropical Horticulture Research, Hainan Academy of Agricultural Science, Haikou, China.

ABSTRACT

Mannitol or sorbitol was added into the Murashige and Skoog (MS) medium contain-ing certain concentrations of 6-Benzyladenine (BA) which was used to induce adventi-tious buds of Echinacea purpurea L. Results showed that the induced adventitious buds growing from medium added with 15 g·L-1 mannitol or sorbitol of the same con-centration were more consistent in height. The regeneration rates in MS medium containing 0.2 mg·L-1 BA and 15 g·L-1 mannitol were increased, while in MS medium containing 0.2 and 0.5 mg·L-1 BA, and 15 g·L-1 sorbitol, the regeneration rates were suppressed. On the other hand, genotype of explants and the concentration of BA in-fluenced the incidence of hyperhydricity, and the hyperhydricity of regenerated buds was more severe when the petiole explants were inoculated on medium with 15 g·L-1 mannitol or 15 g·L-1 sorbitol. The present study offers new possibility to the production of uniform plantlets for commercial cultivation in this important medicinal plant.

KEYWORDS

Echinacea purpurea, Micropropagation, Tissue Culture, Mannitol, Sorbitol, Osmotic Pressure

Share and Cite:

Chen, X. , Li, D. , Yang, Y. and Wu, H. (2016) Mannitol and Sorbitol Improve Uniformity of Adventitious Shoots Regeneration in Echinacea purpurea L. Moench. Journal of Biomedical Science and Engineering, 9, 58-64. doi: 10.4236/jbise.2016.910B008.

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